Literature DB >> 11531703

Polymorphic microsatellites developed by cross-species amplifications in common pheasant breeds.

M Baratti1, A Alberti, M Groenen, T Veenendaal, F D Fulgheri.   

Abstract

Genetic variability was analysed in two common breeds of pheasant (Phasianus colchicus L. 1758) by means of cross-species amplifications of microsatellite loci: 154 chicken, Gallus gallus and 32 turkey, Meleagris gallopavo, primers were tested for amplification of pheasant DNA. Thirty-six primers (25 specific for chicken and 11 for turkey) amplified pheasant DNA. Fifteen markers yielded specific products and were tested for polymorphism. Eight of them (55%) were polymorphic, with an average polymorphism of two alleles per locus. Specific polymerase chain reaction (PCR) products were sequenced; repeats were found in 11 of the 15 markers, although only two loci showed the same repeat and could be homologous to chicken ones.

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Year:  2001        PMID: 11531703     DOI: 10.1046/j.1365-2052.2001.00767.x

Source DB:  PubMed          Journal:  Anim Genet        ISSN: 0268-9146            Impact factor:   3.169


  5 in total

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  5 in total

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