OBJECTIVE: To explore methods suitable for quantitative assessment of the efficacy of chemopreventive intervention. STUDY DESIGN: High-resolution imagery of nuclei from the suprabasal and basal cell layers of sun-damaged skin were recorded. There were 10 cases. A shave biopsy was taken from an area of clearly evident solar keratosis before and after treatment with 2-difluoromethyl-dlornithine (DFMO) and from the colateral forearm, treated with a placebo. A number of karyometric variables were computed and combined to derive marker features that provided a numeric measure of the degree of nuclear deviation from normal. RESULTS:DFMO treatment was effective overall in reducing the degree of nuclear abnormality seen in the biopsies; in 8 of the 10 cases there was a significant improvement. The placebo-treated arm did not show a statistically different abnormality from the untreated arm. CONCLUSION: Karyometric analysis can provide numeric measures that allow documentation of statistically significant regression of actinic keratotic lesions following treatment with DFMO.
RCT Entities:
OBJECTIVE: To explore methods suitable for quantitative assessment of the efficacy of chemopreventive intervention. STUDY DESIGN: High-resolution imagery of nuclei from the suprabasal and basal cell layers of sun-damaged skin were recorded. There were 10 cases. A shave biopsy was taken from an area of clearly evident solar keratosis before and after treatment with 2-difluoromethyl-dlornithine (DFMO) and from the colateral forearm, treated with a placebo. A number of karyometric variables were computed and combined to derive marker features that provided a numeric measure of the degree of nuclear deviation from normal. RESULTS:DFMO treatment was effective overall in reducing the degree of nuclear abnormality seen in the biopsies; in 8 of the 10 cases there was a significant improvement. The placebo-treated arm did not show a statistically different abnormality from the untreated arm. CONCLUSION: Karyometric analysis can provide numeric measures that allow documentation of statistically significant regression of actinic keratotic lesions following treatment with DFMO.
Authors: Peter H Bartels; Michael L Yozwiak; Hubert G Bartels; Yun Liu; Lisa M Hess; David S Alberts Journal: Cancer Epidemiol Biomarkers Prev Date: 2008-06-26 Impact factor: 4.254
Authors: Peter H Bartels; Francisco A R Garcia; Cornelia L Trimble; James Kauderer; John Curtin; Peter C Lim; Lisa M Hess; Steven Silverberg; Richard J Zaino; Michael Yozwiak; Hubert G Bartels; David S Alberts Journal: Gynecol Oncol Date: 2011-12-09 Impact factor: 5.482
Authors: Peter H Bartels; Rodolfo Montironi; Marina Scarpelli; Hubert G Bartels; David S Alberts Journal: Anal Quant Cytol Histol Date: 2009-06 Impact factor: 0.302
Authors: Robert S Krouse; David S Alberts; Anil R Prasad; Michael Yozwiak; Hubert G Bartels; Yun Liu; Peter H Bartels Journal: Anal Quant Cytol Histol Date: 2009-02 Impact factor: 0.302
Authors: Peter H Bartels; Robert S Krouse; Anil R Prasad; Michael Yozwiak; Yun Liu; Hubert G Bartels; David S Alberts Journal: Anal Quant Cytol Histol Date: 2008-12 Impact factor: 0.302