BACKGROUND: Several studies have shown that interleukin (IL)-4 and interferon-gamma (IFN-gamma) are important for the regulation of immunoglobulin E (IgE) production and that IL-18 and IL-12 induce IFN-gamma. OBJECTIVE: IFN-gamma production in response to IL-18 or IL-12 stimulation was investigated in peripheral blood mononuclear cells (PBMCs) of atopic patients with various levels of serum IgE. METHODS: Cytokine production from PBMCs was measured following stimulation with a non-specific stimulator (phytohemagglutinin: PHA), IL-18 or IL-12 in 12 healthy controls and 26 atopic patients with various serum IgE levels. RESULTS: IFN-gamma production by IL-18-stimulated PBMCs was positively correlated with IFN-gamma production by IL-12-stimulated PBMCs (P < 0.05). However some atopic patients showed discrepancy between the levels of IFN-gamma production stimulated by IL-12 and by IL-18. CONCLUSIONS: The results shown here suggest the presence of abnormalities in the IL-12 and/or IL-18 signalling pathways, such as genetic defects in the atopic patients.
BACKGROUND: Several studies have shown that interleukin (IL)-4 and interferon-gamma (IFN-gamma) are important for the regulation of immunoglobulin E (IgE) production and that IL-18 and IL-12 induce IFN-gamma. OBJECTIVE:IFN-gamma production in response to IL-18 or IL-12 stimulation was investigated in peripheral blood mononuclear cells (PBMCs) of atopicpatients with various levels of serum IgE. METHODS: Cytokine production from PBMCs was measured following stimulation with a non-specific stimulator (phytohemagglutinin: PHA), IL-18 or IL-12 in 12 healthy controls and 26 atopicpatients with various serum IgE levels. RESULTS:IFN-gamma production by IL-18-stimulated PBMCs was positively correlated with IFN-gamma production by IL-12-stimulated PBMCs (P < 0.05). However some atopicpatients showed discrepancy between the levels of IFN-gamma production stimulated by IL-12 and by IL-18. CONCLUSIONS: The results shown here suggest the presence of abnormalities in the IL-12 and/or IL-18 signalling pathways, such as genetic defects in the atopicpatients.
Authors: Adrienne G Randolph; Christoph Lange; Edwin K Silverman; Ross Lazarus; Eric S Silverman; Benjamin Raby; Alison Brown; Al Ozonoff; Brent Richter; Scott T Weiss Journal: Am J Hum Genet Date: 2004-08-20 Impact factor: 11.025
Authors: Eun Jin Kim; Won Mok Lee; Jung Sook Ha; Nam Hee Ryoo; Dong Suk Jeon; Jae Ryong Kim Journal: J Korean Med Sci Date: 2006-12 Impact factor: 2.153
Authors: Alessandra Ferraro; Sofia M Buonocore; Philippe Auquier; Isabelle Nicolas; Hugues Wallemacq; Dominique Boutriau; Robbert G van der Most Journal: Hum Vaccin Immunother Date: 2019-10-31 Impact factor: 3.452