BACKGROUND: Allergic asthma is a multifactorial disease for which there is a widely assessed, although poorly understood, genetic involvement. Genome-wide screens reported evidence for linkage of allergic asthma-related phenotypes to several chromosomal locations. Markers on chromosome 19 have been linked to allergic asthma phenotypes in different populations in independent studies. OBJECTIVE: The aim of this study was to perform a genetic linkage analysis on chromosome 19 to search for DNA markers linked to phenotypes related to allergic asthma. METHODS: Using non-parametric multipoint linkage analysis on a total of 22 random DNA markers in 2 stages, a sample of 111 families (542 subjects) from north-eastern Italy, recruited through an asthmatic allergic proband, was investigated. Phenotypes examined were: clinical asthma, total serum elevated IgE, skin prick test positivity, bronchial hyper-responsiveness, and atopy defined as skin prick test positivity and/or elevated IgE. Simulation studies were performed to confirm the significance of the results. RESULTS: A novel linkage of atopy and skin prick test positivity to marker D19S601 (19q13.3) was found. Modest evidence for linkage of atopy, skin prick test positivity, and IgE was also found to marker D19S591 (19p13.3). Simulation analysis for atopy gave an NPL-Z > 3.326 in 2 replicates out of 1000 (P = 0.002) for D19S601, and an NPL-Z > 2.56 in 16 replicates out of 1000 (P = 0.016) for D19S591. CONCLUSIONS: On chromosome 19, suggestive linkage of atopy and skin prick test positivity with marker D19S601 (19q13.3) and modest evidence of linkage of marker D19S591 (19p13.3) to the atopic phenotypes investigated were found. These results suggest that these regions may contain susceptibility loci associated to atopic phenotypes.
BACKGROUND: Allergic asthma is a multifactorial disease for which there is a widely assessed, although poorly understood, genetic involvement. Genome-wide screens reported evidence for linkage of allergic asthma-related phenotypes to several chromosomal locations. Markers on chromosome 19 have been linked to allergic asthma phenotypes in different populations in independent studies. OBJECTIVE: The aim of this study was to perform a genetic linkage analysis on chromosome 19 to search for DNA markers linked to phenotypes related to allergic asthma. METHODS: Using non-parametric multipoint linkage analysis on a total of 22 random DNA markers in 2 stages, a sample of 111 families (542 subjects) from north-eastern Italy, recruited through an asthmatic allergic proband, was investigated. Phenotypes examined were: clinical asthma, total serum elevated IgE, skin prick test positivity, bronchial hyper-responsiveness, and atopy defined as skin prick test positivity and/or elevated IgE. Simulation studies were performed to confirm the significance of the results. RESULTS: A novel linkage of atopy and skin prick test positivity to marker D19S601 (19q13.3) was found. Modest evidence for linkage of atopy, skin prick test positivity, and IgE was also found to marker D19S591 (19p13.3). Simulation analysis for atopy gave an NPL-Z > 3.326 in 2 replicates out of 1000 (P = 0.002) for D19S601, and an NPL-Z > 2.56 in 16 replicates out of 1000 (P = 0.016) for D19S591. CONCLUSIONS: On chromosome 19, suggestive linkage of atopy and skin prick test positivity with marker D19S601 (19q13.3) and modest evidence of linkage of marker D19S591 (19p13.3) to the atopic phenotypes investigated were found. These results suggest that these regions may contain susceptibility loci associated to atopic phenotypes.
Authors: J Matsuzawa; K Sugimura; Y Matsuda; M Takazoe; K Ishizuka; T Mochizuki; S S Seki; O Yoneyama; H Bannnai; K Suzuki; T Honma; H Asakura Journal: Gut Date: 2003-01 Impact factor: 23.059
Authors: Pei-Song Gao; Kenichi Shimizu; Audrey V Grant; Nicholas Rafaels; Lin-Fu Zhou; Sherry A Hudson; Satoshi Konno; Nives Zimmermann; Maria I Araujo; Eduardo V Ponte; Alvaro A Cruz; Masaharu Nishimura; Song-Nan Su; Nobuyuki Hizawa; Terry H Beaty; Rasika A Mathias; Marc E Rothenberg; Kathleen C Barnes; Bruce S Bochner Journal: Eur J Hum Genet Date: 2010-01-20 Impact factor: 4.246
Authors: Rachel A Myers; Blanca E Himes; Christopher R Gignoux; James J Yang; W James Gauderman; Cristina Rebordosa; Jianming Xie; Dara G Torgerson; Albert M Levin; James Baurley; Penelope E Graves; Rasika A Mathias; Isabelle Romieu; Lindsey A Roth; David Conti; Lydiana Avila; Celeste Eng; Hita Vora; Michael A LeNoir; Manuel Soto-Quiros; Jinghua Liu; Juan C Celedón; Joshua M Galanter; Harold J Farber; Rajesh Kumar; Pedro C Avila; Kelley Meade; Denise Serebrisky; Shannon Thyne; William Rodriguez-Cintron; Jose R Rodriguez-Santana; Luisa N Borrell; Robert F Lemanske; Eugene R Bleecker; Deborah A Meyers; Stephanie J London; Kathleen C Barnes; Benjamin A Raby; Fernando D Martinez; Frank D Gilliland; L Keoki Williams; Esteban G Burchard; Scott T Weiss; Dan L Nicolae; Carole Ober Journal: J Allergy Clin Immunol Date: 2012-10-04 Impact factor: 10.793
Authors: Elena S Gusareva; Helena Havelková; Hana Blazková; Marcela Kosarová; Petr Kucera; Vlastimil Král; Daria Salyakina; Bertram Müller-Myhsok; Marie Lipoldová Journal: Immunogenetics Date: 2008-11-18 Impact factor: 2.846