Literature DB >> 11526191

Colorimetric assay for antifungal susceptibility testing of Aspergillus species.

J Meletiadis1, J W Mouton, J F Meis, B A Bouman, J P Donnelly, P E Verweij.   

Abstract

A colorimetric assay for antifungal susceptibility testing of Aspergillus species (Aspergillus fumigatus, Aspergillus flavus, Aspergillus terreus, Aspergillus nidulans, and Aspergillus ustus) is described based on the reduction of the tetrazolium salt 2,3-bis(2-methoxy-4-nitro-5-[(sulphenylamino)carbonyl]-2H-tetrazolium-hydroxide (XTT) in the presence of menadione as an electron-coupling agent. The combination of 200 microg of XTT/ml with 25 microM menadione resulted in a high production of formazan within 2 h of exposure, allowing the detection of hyphae formed by low inocula of 10(2) CFU/ml after 24 h of incubation. Under these settings, the formazan production correlated linearly with the fungal biomass and less-variable concentration effect curves for amphotericin B and itraconazole were obtained.

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Year:  2001        PMID： 11526191      PMCID： PMC88359          DOI： 10.1128/JCM.39.9.3402-3408.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

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Journal:  Prog Histochem Cytochem       Date:  1991

2.  Comparison of a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-t etrazolium hydroxide (XTT) colorimetric method with the standardized National Committee for Clinical Laboratory Standards method of testing clinical yeast isolates for susceptibility to antifungal agents.

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Journal:  J Clin Microbiol       Date:  1998-05       Impact factor: 5.948

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Journal:  Infect Dis Clin North Am       Date:  1993-06       Impact factor: 5.982

4.  Comparison of NCCLS and 3-(4,5-dimethyl-2-Thiazyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) methods of in vitro susceptibility testing of filamentous fungi and development of a new simplified method.

Authors:  J Meletiadis; J F Meis; J W Mouton; J P Donnelly; P E Verweij
Journal:  J Clin Microbiol       Date:  2000-08       Impact factor: 5.948

5.  Susceptibility testing of Candida albicans and Aspergillus species by a simple microtiter menadione-augmented 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay.

Authors:  B Jahn; E Martin; A Stueben; S Bhakdi
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

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Journal:  Prog Histochem Cytochem       Date:  1976

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Authors:  S M Levitz; R D Diamond
Journal:  J Infect Dis       Date:  1985-11       Impact factor: 5.226

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Journal:  J Immunol Methods       Date:  1991-09-13       Impact factor: 2.303

9.  Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines.

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Journal:  Cancer Res       Date:  1988-09-01       Impact factor: 12.701

10.  A simplified new assay for assessment of fungal cell damage with the tetrazolium dye, (2,3)-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carboxanil ide (XTT).

Authors:  T Meshulam; S M Levitz; L Christin; R D Diamond
Journal:  J Infect Dis       Date:  1995-10       Impact factor: 5.226
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Journal:  Antimicrob Agents Chemother       Date:  2003-11       Impact factor: 5.191

Review 2.  In vitro analyses, animal models, and 60 clinical cases of invasive Aspergillus terreus infection.

Authors:  William J Steinbach; John R Perfect; Wiley A Schell; Thomas J Walsh; Daniel K Benjamin
Journal:  Antimicrob Agents Chemother       Date:  2004-09       Impact factor: 5.191

3.  Functional assignment of YvgO, a novel set of purified and chemically characterized proteinaceous antifungal variants produced by Bacillus thuringiensis SF361.

Authors:  David C Manns; John J Churey; Randy W Worobo
Journal:  Appl Environ Microbiol       Date:  2012-02-03       Impact factor: 4.792

4.  Human leukocytes kill Aspergillus nidulans by reactive oxygen species-independent mechanisms.

Authors:  Stefanie S V Henriet; Peter W M Hermans; Paul E Verweij; Elles Simonetti; Steven M Holland; Janyce A Sugui; Kyung J Kwon-Chung; Adilia Warris
Journal:  Infect Immun       Date:  2010-11-15       Impact factor: 3.441

5.  Albumin Enhances Caspofungin Activity against Aspergillus Species by Facilitating Drug Delivery to Germinating Hyphae.

Authors:  Petros Ioannou; Aggeliki Andrianaki; Tonia Akoumianaki; Irene Kyrmizi; Nathaniel Albert; David Perlin; George Samonis; Dimitrios P Kontoyiannis; Georgios Chamilos
Journal:  Antimicrob Agents Chemother       Date:  2015-12-07       Impact factor: 5.191

6.  Rapid susceptibility testing of medically important zygomycetes by XTT assay.

Authors:  Charalampos Antachopoulos; Joseph Meletiadis; Emmanuel Roilides; Tin Sein; Thomas J Walsh
Journal:  J Clin Microbiol       Date:  2006-02       Impact factor: 5.948

7.  Lovastatin has significant activity against zygomycetes and interacts synergistically with voriconazole.

Authors:  Georgios Chamilos; Russell E Lewis; Dimitrios P Kontoyiannis
Journal:  Antimicrob Agents Chemother       Date:  2006-01       Impact factor: 5.191

8.  Endoplasmic reticulum localized PerA is required for cell wall integrity, azole drug resistance, and virulence in Aspergillus fumigatus.

Authors:  Dawoon Chung; Arsa Thammahong; Kelly M Shepardson; Sara J Blosser; Robert A Cramer
Journal:  Mol Microbiol       Date:  2014-05-09       Impact factor: 3.501

9.  Differential fungicidal activities of amphotericin B and voriconazole against Aspergillus species determined by microbroth methodology.

Authors:  Joseph Meletiadis; Charalampos Antachopoulos; Theodouli Stergiopoulou; Spyros Pournaras; Emmanuel Roilides; Thomas J Walsh
Journal:  Antimicrob Agents Chemother       Date:  2007-06-18       Impact factor: 5.191

10.  Hypoxia enhances innate immune activation to Aspergillus fumigatus through cell wall modulation.

Authors:  Kelly M Shepardson; Lisa Y Ngo; Vishukumar Aimanianda; Jean-Paul Latgé; Bridget M Barker; Sara J Blosser; Yoichiro Iwakura; Tobias M Hohl; Robert A Cramer
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