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Literature DB >> 11526177

Isolation and characterization of a species-specific DNA fragment for identification of Candida (Torulopsis) glabrata by PCR.

K Becker¹, D Badehorn, B Keller, M Schulte, K H Böhm, G Peters, W Fegeler.

Abstract

A PCR specific for Candida glabrata that amplifies a mitochondrial rRNA gene fragment was developed by analysis of C. glabrata-specific agarose gel bands, which were generated by arbitrarily primed PCR. The expected PCR product was successfully amplified with genomic DNA from 95 C. glabrata isolates but not from a number of other fungal isolates.

Entities: Chemical Species

Mesh：

Substances：
DNA Primers
DNA, Fungal

Year: 2001 PMID： 11526177 PMCID： PMC88345 DOI： 10.1128/JCM.39.9.3356-3359.2001

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

22 in total

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Journal: J Clin Microbiol Date: 1999-03 Impact factor: 5.948

3. Sequence and secondary structure of the mitochondrial small-subunit rRNA V4, V6, and V9 domains reveal highly species-specific variations within the genus Agrocybe.

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4. Detection of a wide range of medically important fungi by the polymerase chain reaction.

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Journal: J Med Microbiol Date: 1994-05 Impact factor: 2.472

5. Controlled study of fluconazole in the prevention of fungal infections in neutropenic patients with haematological malignancies and bone marrow transplant recipients.

Authors: M E Ellis; H Clink; P Ernst; M A Halim; A Padmos; D Spence; M Kalin; S M Hussain Qadri; J Burnie; W Greer
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6. Detection and differentiation of fungi in clinical specimens using polymerase chain reaction (PCR) amplification and restriction enzyme analysis.

Authors: R L Hopfer; P Walden; S Setterquist; W E Highsmith
Journal: J Med Vet Mycol Date: 1993

7. Molecular cloning and analysis of the nuclear gene MRP-L6 coding for a putative mitochondrial ribosomal protein from Saccharomyces cerevisiae.

Authors: R Harrer; S Schwank; H J Schüller; E Schweizer
Journal: Curr Genet Date: 1993 Jul-Aug Impact factor: 3.886

Isolation and characterization of a species-specific DNA fragment for identification of Candida (Torulopsis) glabrata by PCR.

1. A randomly amplified polymorphic DNA marker specific for the Bacillus cereus group is diagnostic for Bacillus anthracis.

2. Evaluation of two commercial kits and arbitrarily primed PCR for identification and differentiation of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, and Haemophilus paraphrophilus.

3. Sequence and secondary structure of the mitochondrial small-subunit rRNA V4, V6, and V9 domains reveal highly species-specific variations within the genus Agrocybe.

4. Detection of a wide range of medically important fungi by the polymerase chain reaction.

5. Controlled study of fluconazole in the prevention of fungal infections in neutropenic patients with haematological malignancies and bone marrow transplant recipients.

6. Detection and differentiation of fungi in clinical specimens using polymerase chain reaction (PCR) amplification and restriction enzyme analysis.

7. Molecular cloning and analysis of the nuclear gene MRP-L6 coding for a putative mitochondrial ribosomal protein from Saccharomyces cerevisiae.

8. Epidemiological shifts in opportunistic and nosocomial Candida infections: mycological aspects.

Review 9. Importance of Candida species other than C. albicans as pathogens in oncology patients.

10. Diagnostic use of PCR for detection of Pneumocystis carinii in oral wash samples.

1. Systematic survey of nonspecific agglutination by Candida spp. in latex assays.