| Literature DB >> 11526152 |
K W Hart1, O M Williams, N Thelwell, A N Fiander, T Brown, L K Borysiewicz, C M Gelder.
Abstract
We report the development of a novel detection and typing methodology for human papillomaviruses (HPV) based on real-time PCR with the self-probing fluorescent primers known as Scorpions. This technique is quick, simple, specific, sensitive, and capable of estimating viral load per cell. We report the results of over 100 typing reactions performed on cell lines, biopsies, and cervical cytobrush samples which, when compared to the current reference HPV detection and typing technique, present a kappa value of 0.89. We further report preliminary data suggesting a relationship between viral load per cell and grade of cervical disease.Entities:
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Year: 2001 PMID： 11526152 PMCID： PMC88320 DOI： 10.1128/JCM.39.9.3204-3212.2001
Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948