Literature DB >> 11523218

Human vascular endothelial growth factor cDNA cloning and expression in osteoblasts.

C Yang1, S Yang, S Qu.   

Abstract

Human vascular endothelial growth factor (VEGF) cDNA was amplified by nested polymerase chain reaction method from the HL60 cells. Then a pCD-hVEGF165 recombinant plasmid was constructed. Rabbit osteoblasts were transfected with pCD-hVEGF165 plasmid by lipofectin mediated gene transfer. The transient expressive results were detected by immunohistochemical method. It was observed that the expression of human VEGF gene was detected 72 h after transfecting distinctly.

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Year:  2001        PMID： 11523218     DOI： 10.1007/BF02888077

Source DB:  PubMed          Journal:  J Tongji Med Univ        ISSN： 0257-716X


  12 in total

Review 1.  Biology of bone endothelial cells.

Authors:  E A Streeten; M L Brandi
Journal:  Bone Miner       Date:  1990-08

2.  Anabolic effects of 1,25-dihydroxyvitamin D3 on osteoblasts are enhanced by vascular endothelial growth factor produced by osteoblasts and by growth factors produced by endothelial cells.

Authors:  D S Wang; M Miura; H Demura; K Sato
Journal:  Endocrinology       Date:  1997-07       Impact factor: 4.736

3.  Vascular endothelial growth factor is a secreted angiogenic mitogen.

Authors:  D W Leung; G Cachianes; W J Kuang; D V Goeddel; N Ferrara
Journal:  Science       Date:  1989-12-08       Impact factor: 47.728

4.  Treatment of acute limb ischemia by intramuscular injection of vascular endothelial growth factor gene.

Authors:  Y Tsurumi; M Kearney; D Chen; M Silver; S Takeshita; J Yang; J F Symes; J M Isner
Journal:  Circulation       Date:  1997-11-04       Impact factor: 29.690

5.  The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing.

Authors:  E Tischer; R Mitchell; T Hartman; M Silva; D Gospodarowicz; J C Fiddes; J A Abraham
Journal:  J Biol Chem       Date:  1991-06-25       Impact factor: 5.157

6.  Clinical evidence of angiogenesis after arterial gene transfer of phVEGF165 in patient with ischaemic limb.

Authors:  J M Isner; A Pieczek; R Schainfeld; R Blair; L Haley; T Asahara; K Rosenfield; S Razvi; K Walsh; J F Symes
Journal:  Lancet       Date:  1996-08-10       Impact factor: 79.321

7.  Constitutive expression of phVEGF165 after intramuscular gene transfer promotes collateral vessel development in patients with critical limb ischemia.

Authors:  I Baumgartner; A Pieczek; O Manor; R Blair; M Kearney; K Walsh; J M Isner
Journal:  Circulation       Date:  1998-03-31       Impact factor: 29.690

8.  Enhanced expression of vascular endothelial growth factor in human SaOS-2 osteoblast-like cells and murine osteoblasts induced by insulin-like growth factor I.

Authors:  D L Goad; J Rubin; H Wang; A H Tashjian; C Patterson
Journal:  Endocrinology       Date:  1996-06       Impact factor: 4.736

9.  Therapeutic angiogenesis. A single intraarterial bolus of vascular endothelial growth factor augments revascularization in a rabbit ischemic hind limb model.

Authors:  S Takeshita; L P Zheng; E Brogi; M Kearney; L Q Pu; S Bunting; N Ferrara; J F Symes; J M Isner
Journal:  J Clin Invest       Date:  1994-02       Impact factor: 14.808

Review 10.  Vascular permeability factor/vascular endothelial growth factor, microvascular hyperpermeability, and angiogenesis.

Authors:  H F Dvorak; L F Brown; M Detmar; A M Dvorak
Journal:  Am J Pathol       Date:  1995-05       Impact factor: 4.307
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  1 in total

1.  Experimental study of vascular endothelial growth factor gene therapy for avascular necrosis of the femoral head.

Authors:  Cao Yang; Shuhua Yang; Jingyuan Du; Jin Li; Weihua Xu; Yufang Xiong
Journal:  J Huazhong Univ Sci Technolog Med Sci       Date:  2003
  1 in total

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