Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter strain 96 (DNA group 11).

A polysaccharide containing D-Manp, L-Fucp (6-deoxygalactopyranose, fucose) and D-GlcpNAc was isolated by mild acid hydrolysis, followed by gel-permeation chromatography, from the lipopolysaccharide derived from Acinetobacter strain 96 (DNA group 11). The structure of the O-antigen was determined by compositional analysis and NMR spectroscopy of the polysaccharide as: [carbohydrate structure see text] A monoclonal antibody obtained after immunization of mice with heat-killed bacteria of Acinetobacter strain 96 was shown to bind to the O-antigen and did not cross-react with any Acinetobacter O-antigen of known structure.