| Literature DB >> 11516485 |
H Heider1, B Ziaja, C Priemer, A Lundkvist, J Neyts, D H Krüger, R Ulrich.
Abstract
A protocol for chemiluminescence detection of hantaviral antigens in infected cell foci is described. This focus detection is based on the conversion of a substrate into a luminescent product by peroxidase-antibody conjugates; the emitted light of infected cell foci can easily be recorded by autoradiography or video imaging providing a hard copy for documentation. The main advantage of this method as compared to conventional immunochemical staining is a higher detection sensitivity due to the inherent magnification effect of luminescence causing an obvious boost in focal image and intensity. This enables reduction of (i) incubation time of virus-infected cells and (ii) amount of needed antibody for antigen detection in foci. This method is applied to a chemiluminescence focus reduction assay for the serotyping of hantavirus-specific neutralising antibodies in infected persons and for the determination of activity of antiviral agents against hantavirus.Entities:
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Year: 2001 PMID： 11516485 DOI： 10.1016/s0166-0934(01)00314-7
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014