A short hypoosmotic swelling test for the prediction of boar sperm fertility.

This study was designed to explore the relationship between the ejaculate response to a hypoosmotic swelling test (HOST) and in vivo fertility in a group of 38 boars The hypoosmotic test used was a modification of the HOST that involves a shorter incubation time (5 vs 120 min) and lower osmotic pressure (75 vs 150 mOsm/kg). Ejaculates containing less than 20% abnormal spermatozoa were selected and checked for percentage of motility, percentage of normal acrosomes, percentage of short ORT and percentage of cells showing positive short HOST (sHOST) results Two hundred eightyeight sows were inseminated to obtain in vivo fertility and prolificacy data. No differences were shown between technicians in the sHOST results obtained. Significant differences were recorded between boars in sHOST results (p < 0.002). Only the sHOST result presented a significant correlation with in vivo fertility (r = 0.43, p < 0.01). Short HOST data significantly improved fertility prediction of routine semen analysis tests. Unlike motility and acrosomal status, sHOST values showed a significant decrease when fresh ejaculates (37 degrees C) were stored for 24 h at 15 degrees C, indicating possible damage due to cold shock.