Mechanical fluid flow enhances high-affinity-IgE-receptor-mediated secretion by mast cells adherent to fibronectin.

We demonstrated the effect of interaction between mechanical fluid flow and the extracellular matrix on high-affinity-IgE-receptor (FcepsilonRI)-mediated secretion by mast cells (cell line MC/9). Mechanical fluid flow did not affect secretion by the cells in the absence of exogenou agonists. Mechanical fluid flow did not affect secretion by cells adherent tofibronectin in the absence of exogenous agonists, but it did enhance FcepsilonRI-mediated secretion by cells adherent tofibronectin. These finding indicate that fluid-flow-induced enhancement of secretion by mast cells requires both exogenous agonists and interaction between integrins and the extracellular matrix. The present study is thefirst to demonstrate that mechanical fluid flow enhances FcepsilonRI-mediated secretion by a mast cell line adherent tofibronectin.