Literature DB >> 11515373

Development of simple sequence repeat markers from bacterial artificial chromosomes without subcloning.

X Qi1, S Lindup, T S Pittaway, S Allouis, M D Gale, K M Devos.   

Abstract

Simple sequence repeats (SSRs) were isolated from pearl millet bacterial artificial clones (BACs) without any subcloning steps. SSR sequences were targeted using 3' end-anchored SSR primers. Flanking sequences were isolated by suppression PCR. In this pilot study, 25 SSR markers have been developed from 40 BAC pools, comprising a total of 384 clones. This novel way to develop new markers has the added advantage that mapping the SSR markers will anchor individual BACs to the genetic maps and, thus, facilitate the construction of BAC contigs.

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Year:  2001        PMID： 11515373     DOI： 10.2144/01312st08

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  23 in total

1.  Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs, SSRs, RAPDs, and SRAPs.

Authors:  H Budak; R C Shearman; I Parmaksiz; I Dweikat
Journal:  Theor Appl Genet       Date:  2004-03-16       Impact factor: 5.699

2.  Evolutionary dynamics of cycle length in pearl millet: the role of farmer's practices and gene flow.

Authors:  Ghayas Lakis; Athman Maï Ousmane; Douka Sanoussi; Abdoulaye Habibou; Mahamane Badamassi; Françoise Lamy; Naino Jika; Ramatou Sidikou; Toudou Adam; Aboubakry Sarr; Anne Luxereau; Thierry Robert
Journal:  Genetica       Date:  2012-02-12       Impact factor: 1.082

3.  Development of SCAR marker associated with downy mildew disease resistance in pearl millet (Pennisetum glaucum L.).

Authors:  Sudisha Jogaiah; R G Sharathchandra; Niranjan Raj; A B Vedamurthy; H Shekar Shetty
Journal:  Mol Biol Rep       Date:  2014-08-26       Impact factor: 2.316

4.  SSCP-SNP in pearl millet--a new marker system for comparative genetics.

Authors:  I Bertin; J H Zhu; M D Gale
Journal:  Theor Appl Genet       Date:  2005-04-05       Impact factor: 5.699

5.  Diversity of wild and cultivated pearl millet accessions (Pennisetum glaucum [L.] R. Br.) in Niger assessed by microsatellite markers.

Authors:  Cedric Mariac; Viviane Luong; Issoufou Kapran; Aïssata Mamadou; Fabrice Sagnard; Monique Deu; Jacques Chantereau; Bruno Gerard; Jupiter Ndjeunga; Gilles Bezançon; Jean-Louis Pham; Yves Vigouroux
Journal:  Theor Appl Genet       Date:  2006-10-18       Impact factor: 5.699

6.  Development of a molecular linkage map of pearl millet integrating DArT and SSR markers.

Authors:  A Supriya; S Senthilvel; T Nepolean; K Eshwar; V Rajaram; R Shaw; C T Hash; A Kilian; R C Yadav; M L Narasu
Journal:  Theor Appl Genet       Date:  2011-04-08       Impact factor: 5.699

7.  An integrated genetic map and a new set of simple sequence repeat markers for pearl millet, Pennisetum glaucum.

Authors:  X Qi; T S Pittaway; S Lindup; H Liu; E Waterman; F K Padi; C T Hash; J Zhu; M D Gale; K M Devos
Journal:  Theor Appl Genet       Date:  2004-08-18       Impact factor: 5.699

8.  Phylogeny and origin of pearl millet (Pennisetum glaucum [L.] R. Br) as revealed by microsatellite loci.

Authors:  Ibrahima Oumar; Cédric Mariac; Jean-Louis Pham; Yves Vigouroux
Journal:  Theor Appl Genet       Date:  2008-05-27       Impact factor: 5.699

9.  Genomic resources for gene discovery, functional genome annotation, and evolutionary studies of maize and its close relatives.

Authors:  Chao Wang; Xue Shi; Lin Liu; Haiyan Li; Jetty S S Ammiraju; David A Kudrna; Wentao Xiong; Hao Wang; Zhaozhao Dai; Yonglian Zheng; Jinsheng Lai; Weiwei Jin; Joachim Messing; Jeffrey L Bennetzen; Rod A Wing; Meizhong Luo
Journal:  Genetics       Date:  2013-09-13       Impact factor: 4.562

10.  Pearl millet [Pennisetum glaucum (L.) R. Br.] consensus linkage map constructed using four RIL mapping populations and newly developed EST-SSRs.

Authors:  Vengaldas Rajaram; Thirunavukkarasu Nepolean; Senapathy Senthilvel; Rajeev K Varshney; Vincent Vadez; Rakesh K Srivastava; Trushar M Shah; Ambawat Supriya; Sushil Kumar; Basava Ramana Kumari; Amindala Bhanuprakash; Mangamoori Lakshmi Narasu; Oscar Riera-Lizarazu; Charles Thomas Hash
Journal:  BMC Genomics       Date:  2013-03-09       Impact factor: 3.969
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