Accelerating effect of 12-keto oleic acid on lipid peroxide and fluorescent productions in mouse liver homogenate.

12-Keto oleic acid is generally considered to be a secondary degradation product of peroxides produced by lipid peroxidation. The acid was added to a mouse liver homogenate or to a bovine serum albumin and its influences on lipid peroxidation and fluorescent production were investigated and compared with other acids. Just as with vitamin E deficiency, 12-keto oleic acid was shown to increase lipid peroxide formation and fluorescence productiod directly and indirectly. The increase of lipid peroxide formation was caused directly through the increase of the free radical production, and indirectly through change of the biomembrane structure. The fluorescence production increase was caused directly by reaction of the 12-keto oleic acid itself, and indirectly by acceleration of the lipid peroxidation.