Literature DB >> 11513851

A sensitive filter retention assay for the detection of PrP(Sc) and the screening of anti-prion compounds.

K F Winklhofer1, F U Hartl, J Tatzelt.   

Abstract

A hallmark of prion diseases is the accumulation of an abnormally folded prion protein, denoted PrP(Sc). Here we describe a new and highly sensitive method for the detection of PrP(Sc) in brain and other tissue samples that utilizes both PrP(Sc) diagnostic criteria in combination; protease resistance and aggregation. Upon filtration of tissue extracts derived from scrapie- or bovine spongiform encephalopathy-infected animals, PrP(Sc) is retained and detected on the membranes. Laborious steps such as SDS-PAGE and Western blotting are avoided with concomitant gain in sensitivity and reliability. The new procedure also proved useful in a screen for anti-prion compounds in a scrapie-infected cell culture model.

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Year:  2001        PMID： 11513851     DOI： 10.1016/s0014-5793(01)02692-8

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

1.  Detergents modify proteinase K resistance of PrP Sc in different transmissible spongiform encephalopathies (TSEs).

Authors:  Johanna Breyer; Wiebke M Wemheuer; Arne Wrede; Catherine Graham; Sylvie L Benestad; Bertram Brenig; Jürgen A Richt; Walter J Schulz-Schaeffer
Journal:  Vet Microbiol       Date:  2011-12-14       Impact factor: 3.293

2.  Systematic identification of antiprion drugs by high-throughput screening based on scanning for intensely fluorescent targets.

Authors:  Uwe Bertsch; Konstanze F Winklhofer; Thomas Hirschberger; Jan Bieschke; Petra Weber; F Ulrich Hartl; Paul Tavan; Jörg Tatzelt; Hans A Kretzschmar; Armin Giese
Journal:  J Virol       Date:  2005-06       Impact factor: 5.103

3.  New inhibitors of scrapie-associated prion protein formation in a library of 2000 drugs and natural products.

Authors:  David A Kocisko; Gerald S Baron; Richard Rubenstein; Jiancao Chen; Salomon Kuizon; Byron Caughey
Journal:  J Virol       Date:  2003-10       Impact factor: 5.103

Review 4.  The synaptic pathology of alpha-synuclein aggregation in dementia with Lewy bodies, Parkinson's disease and Parkinson's disease dementia.

Authors:  Walter J Schulz-Schaeffer
Journal:  Acta Neuropathol       Date:  2010-06-20       Impact factor: 17.088

5.  Rapid, high-throughput detection of PrPSc by 96-well immunoassay.

Authors:  Michelle L Kramer; Jason C Bartz
Journal:  Prion       Date:  2009-01-13       Impact factor: 3.931

6.  Similarities between forms of sheep scrapie and Creutzfeldt-Jakob disease are encoded by distinct prion types.

Authors:  Wiebke M Wemheuer; Sylvie L Benestad; Arne Wrede; Ulf Schulze-Sturm; Wilhelm E Wemheuer; Uwe Hahmann; Joanna Gawinecka; Ekkehard Schütz; Inga Zerr; Bertram Brenig; Bjørn Bratberg; Olivier Andréoletti; Walter J Schulz-Schaeffer
Journal:  Am J Pathol       Date:  2009-10-22       Impact factor: 4.307

Review 7.  Prion protein conversion in vitro.

Authors:  Surachai Supattapone
Journal:  J Mol Med (Berl)       Date:  2004-03-10       Impact factor: 4.599

8.  Fluorescent filter-trap assay for amyloid fibril formation kinetics in complex solutions.

Authors:  Irem Nasir; Sara Linse; Celia Cabaleiro-Lago
Journal:  ACS Chem Neurosci       Date:  2015-05-20       Impact factor: 4.418

9.  Formation of Toxic Oligomeric Assemblies of RNA-binding Protein: Musashi in Alzheimer's disease.

Authors:  Urmi Sengupta; Mauro Montalbano; Salome McAllen; Gerard Minuesa; Michael Kharas; Rakez Kayed
Journal:  Acta Neuropathol Commun       Date:  2018-10-26       Impact factor: 7.801
  9 in total

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