Literature DB >> 11511811

Molecular cloning and expression analysis of a mouse UDP-GlcNAc:Gal(beta1-4)Glc(NAc)-R beta1,3-N-acetylglucosaminyltransferase homologous to Drosophila melanogaster Brainiac and the beta1,3-galactosyltransferase family.

S Egan1, B Cohen, M Sarkar, Y Ying, S Cohen, N Singh, W Wang, G Flock, T Goh, H Schachter.   

Abstract

We have isolated a murine cDNA coding for a beta1,3-N-acetylglucosaminyltransferase enzyme ( beta3GnT). This enzyme is similar in sequence to Drosophila melanogaster Brainiac and to the murine and human beta1,3-galactosyltransferase family of proteins. The mouse beta 3GnT protein is 397 amino acids in length and contains 7 cysteine residues that are conserved in the human orthologue. beta 3GnT is a type II membrane protein localized to the Golgi apparatus. Enzyme assays with recombinant mouse beta 3GnT reveal that it has a preference for acceptors with Gal(beta1-4)Glc(NAc) at the non-reducing termini. Proton NMR analysis of product showed incorporation of GlcNAc in beta1,3 linkage to the terminal Gal of Gal(beta1-4)Glc(beta1-O-benzyl). Northern blot analysis revealed the presence of a single 3.0[emsp4 ]kb transcript in all adult mouse and human organs tested, with highest levels in the kidney, liver, heart and placenta. The beta 3GnT gene is also expressed in a number of tumor cell lines. The human orthologue of beta 3GnT is located on chromosome 2pl5.

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Year:  2000        PMID： 11511811     DOI： 10.1023/a:1010921313314

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  34 in total

1.  Fringe is a glycosyltransferase that modifies Notch.

Authors:  D J Moloney; V M Panin; S H Johnston; J Chen; L Shao; R Wilson; Y Wang; P Stanley; K D Irvine; R S Haltiwanger; T F Vogt
Journal:  Nature       Date:  2000-07-27       Impact factor: 49.962

2.  Secreted fringe-like signaling molecules may be glycosyltransferases.

Authors:  Y P Yuan; J Schultz; M Mlodzik; P Bork
Journal:  Cell       Date:  1997-01-10       Impact factor: 41.582

3.  Expression cloning of rat cDNA encoding UDP-galactose:GD2 beta1,3-galactosyltransferase that determines the expression of GD1b/GM1/GA1.

Authors:  H Miyazaki; S Fukumoto; M Okada; T Hasegawa; K Furukawa
Journal:  J Biol Chem       Date:  1997-10-03       Impact factor: 5.157

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Cloning, expression, and characterization of a novel UDP-galactose:beta-N-acetylglucosamine beta1,3-galactosyltransferase (beta3Gal-T5) responsible for synthesis of type 1 chain in colorectal and pancreatic epithelia and tumor cells derived therefrom.

Authors:  S Isshiki; A Togayachi; T Kudo; S Nishihara; M Watanabe; T Kubota; M Kitajima; N Shiraishi; K Sasaki; T Andoh; H Narimatsu
Journal:  J Biol Chem       Date:  1999-04-30       Impact factor: 5.157

6.  Differential expression of beta1,3galactosyltransferases in human colon cells derived from adenocarcinomas or normal mucosa.

Authors:  A Bardoni; M Valli; M Trinchera
Journal:  FEBS Lett       Date:  1999-05-14       Impact factor: 4.124

7.  Cloning of a human UDP-galactose:2-acetamido-2-deoxy-D-glucose 3beta-galactosyltransferase catalyzing the formation of type 1 chains.

Authors:  F Kolbinger; M B Streiff; A G Katopodis
Journal:  J Biol Chem       Date:  1998-01-02       Impact factor: 5.157

8.  Mucin synthesis. The action of pig gastric mucosal UDP-GlcNAc:Gal beta 1-3(R1)GalNAc-R2 (GlcNAc to Gal) beta 3-N-acetylglucosaminyltransferase on high molecular weight substrates.

Authors:  I Brockhausen; J Orr; H Schachter
Journal:  Can J Biochem Cell Biol       Date:  1984-11

9.  Purification and characterization of UDP-GlcNAc:Gal beta 1-4Glc(NAc) beta-1,3-N-acetylglucosaminyltransferase (poly-N-acetyllactosamine extension enzyme) from calf serum.

Authors:  H Kawashima; K Yamamoto; T Osawa; T Irimura
Journal:  J Biol Chem       Date:  1993-12-25       Impact factor: 5.157

10.  Organization and localization to chromosome 5 of the human UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I gene.

Authors:  E Hull; M Sarkar; M P Spruijt; J W Höppener; R Dunn; H Schachter
Journal:  Biochem Biophys Res Commun       Date:  1991-04-30       Impact factor: 3.575
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  1 in total

1.  B3GNT3 acts as a carcinogenic factor in endometrial cancer via facilitating cell growth, invasion and migration through regulating RhoA/RAC1 pathway-associated markers.

Authors:  Ji-Shui Wang; Fang Ruan; Li-Zhu Guo; Feng-Ge Wang; Fu-Ling Wang; Hong-Min An
Journal:  Genes Genomics       Date:  2021-03-08       Impact factor: 1.839
  1 in total

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