H Ding1, R Peng, J Yu. 1. Department of Pharmacology, Medical College of Wuhan University, Wuhan 430071, China.
Abstract
OBJECTIVE: To investigate the modulation of Angelica Sinensis Polysaccharides on the expression of nitric oxide synthase and Bax, Bcl-2 in the liver of immunological liver injured mice. METHODS: Immunological liver injury was induced by lipopolysaccharide (ip, 2mug/10g) in bacillus calmette-guerin (ip, 1mg/10g, qd/1 d) primed mice. Angelica Sinensis Polysaccharides was administrated to the mice with 30mg/kg, 60mg/kg, respectively. The effects of Angelica Sinensis Polysaccharides on the expression of nitric oxide synthase, Bcl-2, and Bax gene were assessed by immunohistochemistry, and the nitric oxide (NO) production, alanine transaminase (ALT) activity, glutathione S-transferase (GST) activity in serum were determined. RESULTS: The levels of NO, sALT, and sGST were significantly higher in immunological liver-injured mice than controls. The contents of iNOS and Bax were 17.8 times (P<0.001) and 1.31 times (P<0.05) of control values, respectively. cNOS expression was not obviously changed, and no Bcl-2 expression was found in immunological liver-injured mice. Angelica Sinensis Polysaccharides of 30mg/kg could reduce the levels of NO, sALT, and sGST by 24.6%, 40.8%, and 18.4%, respectively; the expression of iNOS and Bax decrease by 84.2% and 37.1%; and the expression of cNOS, Bcl-2 increase by 66.8% and 3.38 times, respectively. The influence of Angelica Sinensis Polysaccharides of 60mg/kg was not so obvious as that of 30mg/kg on sGST, NO, cNOS, Bax, and Bcl-2, but was more effective on sALT and iNOS. CONCLUSIONS: NO production may play a role in the LPS-induced hepatotoxicity. Angelica Sinensis Polysaccharides can alleviate the immune liver injury by modulating the expression of cNOS, iNOS, Bax, Bcl-2.
OBJECTIVE: To investigate the modulation of Angelica SinensisPolysaccharides on the expression of nitric oxide synthase and Bax, Bcl-2 in the liver of immunological liver injured mice. METHODS: Immunological liver injury was induced by lipopolysaccharide (ip, 2mug/10g) in bacillus calmette-guerin (ip, 1mg/10g, qd/1 d) primed mice. Angelica SinensisPolysaccharides was administrated to the mice with 30mg/kg, 60mg/kg, respectively. The effects of Angelica SinensisPolysaccharides on the expression of nitric oxide synthase, Bcl-2, and Bax gene were assessed by immunohistochemistry, and the nitric oxide (NO) production, alanine transaminase (ALT) activity, glutathione S-transferase (GST) activity in serum were determined. RESULTS: The levels of NO, sALT, and sGST were significantly higher in immunological liver-injured mice than controls. The contents of iNOS and Bax were 17.8 times (P<0.001) and 1.31 times (P<0.05) of control values, respectively. cNOS expression was not obviously changed, and no Bcl-2 expression was found in immunological liver-injured mice. Angelica SinensisPolysaccharides of 30mg/kg could reduce the levels of NO, sALT, and sGST by 24.6%, 40.8%, and 18.4%, respectively; the expression of iNOS and Bax decrease by 84.2% and 37.1%; and the expression of cNOS, Bcl-2 increase by 66.8% and 3.38 times, respectively. The influence of Angelica SinensisPolysaccharides of 60mg/kg was not so obvious as that of 30mg/kg on sGST, NO, cNOS, Bax, and Bcl-2, but was more effective on sALT and iNOS. CONCLUSIONS: NO production may play a role in the LPS-induced hepatotoxicity. Angelica SinensisPolysaccharides can alleviate the immune liver injury by modulating the expression of cNOS, iNOS, Bax, Bcl-2.
Authors: George Binh Lenon; Chun Guang Li; Charlie Changli Xue; Francis Chung Kong Thien; David Frederick Story Journal: Evid Based Complement Alternat Med Date: 2006-11-27 Impact factor: 2.629