| Literature DB >> 11506464 |
J Metzger1, M Blobner, P B Luppa.
Abstract
We present the establishment of a sensitive immunoassay for the determination of alpha1-acid glycoprotein (orosomucoid, AGP) in rat serum. The assay is based upon antigen capture by surface-immobilized specific polyclonal rabbit anti-AGP antibodies with biotinylated rat AGP (rAGP) as the tracer, and formatted as competitive enzyme immunoassay. Signaling is performed by streptavidin-conjugated horseradish peroxidase. Enzyme activity is quantified by an enhanced chemiluminometric method, allowing the sensitive detection of rAGP serum levels in small sample volumes.Entities:
Mesh：
Substances：
Year: 2001 PMID： 11506464 DOI： 10.1515/CCLM.2001.086
Source DB: PubMed Journal: Clin Chem Lab Med ISSN： 1434-6621 Impact factor: 3.694