Literature DB >> 11499931

Genetic transformation of Trametes versicolor to phleomycin resistance with the dominant selectable marker shble.

K Bartholomew1, G Dos Santos, T Dumonceaux, T Charles, F Archibald.   

Abstract

We have developed a stable, DNA-mediated transformation system for the white-rot basidiomycete Trametes versicolor based on the dominant selectable marker shble (phleomycin resistance). We employed a vector containing the selectable marker under control of expression sequences from the basidiomycete Schizophyllum commune and a polyethylene glycol/ CaCl2 protoplast-fusion technique to introduce the transforming DNA. This transformation system generated stable phleomycin-resistant transformants at a frequency of four to seven transformants/microg of transforming DNA.

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Year:  2001        PMID： 11499931     DOI： 10.1007/s002530100635

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN： 0175-7598            Impact factor:   4.813


  4 in total

1.  Establishing molecular tools for genetic manipulation of the pleuromutilin-producing fungus Clitopilus passeckerianus.

Authors:  Sreedhar Kilaru; Catherine M Collins; Amanda J Hartley; Andy M Bailey; Gary D Foster
Journal:  Appl Environ Microbiol       Date:  2009-09-18       Impact factor: 4.792

2.  Investigating dominant selection markers for Coprinopsis cinerea: a carboxin resistance system and re-evaluation of hygromycin and phleomycin resistance vectors.

Authors:  Sreedhar Kilaru; Catherine M Collins; Amanda J Hartley; Claire Burns; Gary D Foster; Andy M Bailey
Journal:  Curr Genet       Date:  2009-07-28       Impact factor: 3.886

3.  Phleomycin increases transformation efficiency and promotes single integrations in Schizophyllum commune.

Authors:  Arend F van Peer; Charissa de Bekker; Arman Vinck; Han A B Wösten; Luis G Lugones
Journal:  Appl Environ Microbiol       Date:  2008-12-29       Impact factor: 4.792

4.  Expression of a manganese peroxidase isozyme 2 transgene in the ethanologenic white rot fungus Phlebia sp. strain MG-60.

Authors:  Yumi Yamasaki; Megumi Yamaguchi; Kenji Yamagishi; Hirofumi Hirai; Ryuichiro Kondo; Ichiro Kamei; Sadatoshi Meguro
Journal:  Springerplus       Date:  2014-11-27
  4 in total

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