W Zhao1, G Zhou, H He. 1. Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Abstract
OBJECTIVE: To further characterize the HBbl7 and Ml viruses isolated from Hainan Province by molecular biology. METHODS: The fragment containing partial El and 3'untranslated region of each of the viral isolate was amplified by RT-PCR, then subcloned. Recombinant was screened and sequenced. Analysis and comparison were carried out. RESULTS: 1.6kb fragment was amplified from HBbl7 and a 1.3kb was from Ml Comparison of sequences showed, in 3'untranslated region the nucleotide homology between HBbl7 and T48, the prototype of Ross River virus, is 99%, between Ml and Sagiyama virus is 98%. In the partial El sequence, the nucleotide (amino acid) homology between HBbl7 and T48 is 99%(99%),between Ml and Sagiyama virus is 97%(99%), and between Ml and Cetah virus is 94% (98%). CONCLUSIONS: Sequence analysis showed HBb17 virus belongs to Ross River virus, Ml virus belongs to Sagiyama virus or Cetah virus.
OBJECTIVE: To further characterize the HBbl7 and Ml viruses isolated from Hainan Province by molecular biology. METHODS: The fragment containing partial El and 3'untranslated region of each of the viral isolate was amplified by RT-PCR, then subcloned. Recombinant was screened and sequenced. Analysis and comparison were carried out. RESULTS: 1.6kb fragment was amplified from HBbl7 and a 1.3kb was from Ml Comparison of sequences showed, in 3'untranslated region the nucleotide homology between HBbl7 and T48, the prototype of Ross River virus, is 99%, between Ml and Sagiyama virus is 98%. In the partial El sequence, the nucleotide (amino acid) homology between HBbl7 and T48 is 99%(99%),between Ml and Sagiyama virus is 97%(99%), and between Ml and Cetah virus is 94% (98%). CONCLUSIONS: Sequence analysis showed HBb17 virus belongs to Ross River virus, Ml virus belongs to Sagiyama virus or Cetah virus.