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Literature DB >> 114985

PaeExo IX: a unique deoxyribonuclease from Pseudomonas aeruginosa active in the presence of EDTA.

Abstract

A new deoxyribonuclease, PaeExo IX, has been purified to electrophoretic homogeneity from extracts of Pseudomonas aeruginosa strain PAO. This enzyme, which is active in the presence of EDTA, is equally efficient in hydrolyzing native and heart-denatured DNA to acid-s-luble products. The enzyme is partially or totally inhibited by the presence of several divalent cations. The active protein has a molecular weight of 1.6 +/- 0.1 x 10(5) and is composed of two nonidentical polypeptides with molecular weights of 78,000 and 69,000.

Entities: Chemical Species

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Year: 1979 PMID： 114985 PMCID： PMC328003 DOI： 10.1093/nar/7.1.167

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

27 in total

1. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors: R G MARTIN; B N AMES
Journal: J Biol Chem Date: 1961-05 Impact factor: 5.157

2. The deoxyribonucleases of Escherichia coli. I. Purification and properties of a phosphodiesterase.

Authors: I R LEHMAN
Journal: J Biol Chem Date: 1960-05 Impact factor: 5.157

3. Isolation and characterization of mutants of Haemophilus influenzae deficient in an adenosine 5'-triphosphate-dependent deoxyribonuclease activity.

Authors: K W Wilcox; H O Smith
Journal: J Bacteriol Date: 1975-05 Impact factor: 3.490

4. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

5. An adenosine triphosphate-dependent deoxyribonuclease from Hemophilus influenzae Rd. 3. Substrate specificity.

Authors: E A Friedman; H O Smith
Journal: J Biol Chem Date: 1972-05-10 Impact factor: 5.157

6. The isolation and characterization from Escherichia coli of an adenosine triphosphate-dependent deoxyribonuclease directed by rec B, C genes.

Authors: M Wright; G Buttin; J Hurwitz
Journal: J Biol Chem Date: 1971-11 Impact factor: 5.157

7. Uncoupling of the recBC ATPase from DNase by DNA crosslinked with psoralen.

Authors: A E Karu; S Linn
Journal: Proc Natl Acad Sci U S A Date: 1972-10 Impact factor: 11.205

8. F116, D3 and G101: temperate bacteriophages of Pseudomonas aeruginosa.

Authors: R V Miller; J M Pemberton; K E Richards
Journal: Virology Date: 1974-06 Impact factor: 3.616

9. NgoII, a restriction endonuclease from Neisseria gonorrhoeae.

Authors: D J Clanton; W S Riggsby; R V Miller
Journal: J Bacteriol Date: 1979-03 Impact factor: 3.490

10. Biochemical and genetic studies of recombination proficiency in Escherichia coli. II. Rec+ revertants caused by indirect suppression of rec- mutations.

Authors: S D Barbour; H Nagaishi; A Templin; A J Clark
Journal: Proc Natl Acad Sci U S A Date: 1970-09 Impact factor: 11.205

4 in total