Literature DB >> 1149246

A rapid gas chromatographic method for the quantitation of underivatised individual free fatty acids in plasma.

D Sampson, W J Hensley.   

Abstract

A rapid method for quantitating individual free fatty acids (FFA) in plasma has been developed. An internal standard is added to plasma, the FFA are extracted into an organic solvent, concentrated and injected into a gas chromatograph. For routine estimations, extraction and assay of a single sample takes about 30 min and up to 30 samples can be assayed in a day. Seven FFA (myristic, palmitic, palmitoleic, stearic, oleic, linoleic and linolenic acids), are routinely quantitated. This method can be used to monitor individual FFA levels, to determine the percentage composition of individual FFA in plasma or to investigate metabolic disorders involving fatty acids, for example Refsum's disease.

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Year:  1975        PMID： 1149246     DOI： 10.1016/0009-8981(75)90391-5

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  7 in total

1.  Sex-related differences in the plasma protein binding of lignocaine and diazepam.

Authors:  P A Routledge; W W Stargel; B B Kitchell; A Barchowsky; D G Shand
Journal:  Br J Clin Pharmacol       Date:  1981-03       Impact factor: 4.335

2.  Heparin administration increases plasma warfarin binding in man.

Authors:  P A Routledge; T D Bjornsson; B B Kitchell; D G Shand
Journal:  Br J Clin Pharmacol       Date:  1979-09       Impact factor: 4.335

3.  Effect of beta-adrenergic stimulation on free fatty acid content in subepicardial and subendocardial layers of the dog heart in situ. Separation and assay by capillary gas chromatography.

Authors:  S Al Makdessi; H Herilier; J L Andrieu; G Faucon
Journal:  Basic Res Cardiol       Date:  1985 Mar-Apr       Impact factor: 17.165

4.  The effects of age and smoking on the plasma protein binding of lignocaine and diazepam.

Authors:  D Davis; S H Grossman; B B Kitchell; D G Shand; P A Routledge
Journal:  Br J Clin Pharmacol       Date:  1985-02       Impact factor: 4.335

5.  Utilization of extracellular lipids by HT29/219 cancer cells in culture.

Authors:  S Pazouki; J D Baty; H M Wallace; C S Coleman
Journal:  Lipids       Date:  1992-11       Impact factor: 1.880

6.  Comparative Serum Fatty Acid Profiles of Captive and Free-Ranging Cheetahs (Acinonyx jubatus) in Namibia.

Authors:  Adrian S W Tordiffe; Bettina Wachter; Sonja K Heinrich; Fred Reyers; Lodewyk J Mienie
Journal:  PLoS One       Date:  2016-12-19       Impact factor: 3.240

Review 7.  A review of odd-chain fatty acid metabolism and the role of pentadecanoic Acid (c15:0) and heptadecanoic Acid (c17:0) in health and disease.

Authors:  Benjamin Jenkins; James A West; Albert Koulman
Journal:  Molecules       Date:  2015-01-30       Impact factor: 4.411
  7 in total

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