Literature DB >> 11485550

Overexpression of matrix metalloproteinase-2 mediates phenotypic transformation of lens epithelial cells.

Y Seomun1, J Kim, E H Lee, C K Joo.   

Abstract

Transforming growth factor-beta (TGF-beta) is known to be a causative factor in pathological fibrosis and the metastasis of cancer cells, through effects on molecules of the extracellular matrix (ECM). We evaluated the influence of TGF-beta(1) on the gene expression of matrix metalloproteinase-2 (MMP-2) in lens epithelial cells (LECs). The results showed that TGF-beta(1) induced the expression of mRNA for MMP-2 in LECs. Subsequently, in order to examine the role of MMP-2, we overexpressed MMP-2 in LECs by stable transfection. The MMP-2-overexpressing LECs showed typical indicators of a myofibroblast-like cell phenotype, such as multiple layers of cells, elongated morphology, and expression of alpha-smooth muscle actin. We also showed that an MMP inhibitor blocked the TGF-beta(1)-induced morphological change in LECs. These results demonstrate that MMP-2 plays a role in the transformation of LECs, which has implications for the pathological fibrosis of these cells.

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Year:  2001        PMID： 11485550      PMCID： PMC1222030          DOI： 10.1042/0264-6021:3580041

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  48 in total

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Authors:  H Y Huang; Y Wen; J C Irwin; J S Kruessel; Y K Soong; M L Polan
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3.  Tissue inhibitor of metalloproteinase-2 (TIMP-2) binds to the catalytic domain of the cell surface receptor, membrane type 1-matrix metalloproteinase 1 (MT1-MMP).

Authors:  S Zucker; M Drews; C Conner; H D Foda; Y A DeClerck; K E Langley; W F Bahou; A J Docherty; J Cao
Journal:  J Biol Chem       Date:  1998-01-09       Impact factor: 5.157

4.  Role of laminin in matrix induction of macrophage urokinase-type plasminogen activator and 92-kDa metalloproteinase expression.

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Journal:  J Biol Chem       Date:  1997-03-28       Impact factor: 5.157

5.  Effects of transforming growth factor-beta1 on renal extracellular matrix components and their regulating proteins.

Authors:  J A Douthwaite; T S Johnson; J L Haylor; P Watson; A M El Nahas
Journal:  J Am Soc Nephrol       Date:  1999-10       Impact factor: 10.121

6.  Stromelysin-3 is induced in tumor/stroma cocultures and inactivated via a tumor-specific and basic fibroblast growth factor-dependent mechanism.

Authors:  B P Mari; I C Anderson; S E Mari; Y Ning; Y Lutz; L Kobzik; M A Shipp
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7.  Dual expression of matrix metalloproteinase-2 and membrane-type 1-matrix metalloproteinase in fibrotic human livers.

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8.  Transforming growth factor-beta 1 promotes contraction of collagen gel by bovine corneal fibroblasts through differentiation of myofibroblasts.

Authors:  H Kurosaka; D Kurosaka; K Kato; Y Mashima; Y Tanaka
Journal:  Invest Ophthalmol Vis Sci       Date:  1998-04       Impact factor: 4.799

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Authors:  K Gohji; M Nomi; I Hara; S Arakawa; S Kamidono
Journal:  Urol Res       Date:  1998

10.  Expression of matrix metalloproteinases during rat skin wound healing: evidence that membrane type-1 matrix metalloproteinase is a stromal activator of pro-gelatinase A.

Authors:  A Okada; C Tomasetto; Y Lutz; J P Bellocq; M C Rio; P Basset
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  17 in total

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Review 2.  Matrix metalloproteinases as therapeutic targets for idiopathic pulmonary fibrosis.

Authors:  Vanessa J Craig; Li Zhang; James S Hagood; Caroline A Owen
Journal:  Am J Respir Cell Mol Biol       Date:  2015-11       Impact factor: 6.914

3.  Matrix metalloproteinase inhibitors suppress transforming growth factor-beta-induced subcapsular cataract formation.

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Journal:  Am J Pathol       Date:  2006-01       Impact factor: 4.307

4.  Matrix metalloproteinase-9-null mice are resistant to TGF-β-induced anterior subcapsular cataract formation.

Authors:  Anna Korol; Giuseppe Pino; Dhruva Dwivedi; Jennifer V Robertson; Paula A Deschamps; Judith A West-Mays
Journal:  Am J Pathol       Date:  2014-05-06       Impact factor: 4.307

5.  Protein expression profiling of lens epithelial cells from Prdx6-depleted mice and their vulnerability to UV radiation exposure.

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6.  Presence of myofibroblasts and expression of matrix metalloproteinase-2 (MMP-2) in ameloblastomas correlate with rupture of the osseous cortical.

Authors:  Eduardo Rodrigues Fregnani; Lays M Sobral; Fabio Abreu Alves; Fernando Augusto Soares; Luis Paulo Kowalski; Ricardo D Coletta
Journal:  Pathol Oncol Res       Date:  2008-12-19       Impact factor: 3.201

7.  Matrix Metalloproteinases as Mediators of Primary and Secondary Cataracts.

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Journal:  Expert Rev Ophthalmol       Date:  2007

8.  Sprouty is a negative regulator of transforming growth factor β-induced epithelial-to-mesenchymal transition and cataract.

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9.  Elevated tropomyosin expression is associated with epithelial-mesenchymal transition of lens epithelial cells.

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10.  Nuclear translocation of myocardin-related transcription factor-A during transforming growth factor beta-induced epithelial to mesenchymal transition of lens epithelial cells.

Authors:  Madhuja Gupta; Anna Korol; Judith A West-Mays
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