Literature DB >> 1147914

A micro method for simultaneous determination of galactosyltransferase and 5'-nucleotidase activities in cell fractions.

L S Freilich, M E Richmond, A C Reppucci, J E Silbert.   

Abstract

Galactosyltransferase and 5'-nucleotidase were assayed in the same reaction mixture, with ovalbumin as exogenous acceptor of (14-C)galactose and with (3-H)AMP as the substrate for the 5'-nucleotidase assay. The substrates and reaction products of either assay had no significant effect on the activity of the other enzyme.

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Year:  1975        PMID： 1147914      PMCID： PMC1165366          DOI： 10.1042/bj1460741

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  8 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Studies on plasma membranes. 3. Mg2+-ATPase,(Na+-K+-Mg2+)-ATPase and 5'-nucleotidase activity of plasma membranes isolated from rat liver.

Authors:  P Emmelot; C J Bos
Journal:  Biochim Biophys Acta       Date:  1966-07-13

3.  Intracellular localization of liver sugar nucleotide glycoprotein glycosyltransferases in a Golgi-rich fraction.

Authors:  H Schachter; I Jabbal; R L Hudgin; L Pinteric; E J McGuire; S Roseman
Journal:  J Biol Chem       Date:  1970-03-10       Impact factor: 5.157

4.  Glycoprotein metabolism: a UDP-galactose-glycoprotein galactosyltransferase of rat serum.

Authors:  R R Wagner; M A Cynkin
Journal:  Biochem Biophys Res Commun       Date:  1971-10-01       Impact factor: 3.575

5.  Glycoprotein biosynthesis: studies on thyroglobulin. Thyroid galactosyltransferase.

Authors:  M J Spiro; R G Spiro
Journal:  J Biol Chem       Date:  1968-12-25       Impact factor: 5.157

6.  Incorporation of D-galactose into glycoproteins.

Authors:  E J McGuire; G W Jourdian; D M Carlson; S Roseman
Journal:  J Biol Chem       Date:  1965-10       Impact factor: 5.157

7.  Golgi fractions prepared from rat liver homogenates. II. Biochemical characterization.

Authors:  J J Bergeron; J H Ehrenreich; P Siekevitz; G E Palade
Journal:  J Cell Biol       Date:  1973-10       Impact factor: 10.539

8.  Isolation and characterization of Golgi membranes from bovine liver.

Authors:  B Fleischer; S Fleischer; H Ozawa
Journal:  J Cell Biol       Date:  1969-10       Impact factor: 10.539
  8 in total
  4 in total

1.  Studies on intracellular transport of secretory proteins in the rat exocrine pancreas. V. Kinetic studies on accelerated transport following caerulein infusion in vivo.

Authors:  W Bieger; J Seybold; H F Kern
Journal:  Cell Tissue Res       Date:  1976-07-26       Impact factor: 5.249

2.  Hen ovomucoid-agarose: A new conjugate for the isolation by affinity chromatography of UDP-galactose: Glycoprotein galactosyl-transferase.

Authors:  B Tadolini; G Hakim
Journal:  Appl Biochem Biotechnol       Date:  1981-09       Impact factor: 2.926

3.  Biosynthesis of chondroitin sulphate by a Golgi-apparatus-enriched preparation from cultures of mouse mastocytoma cells.

Authors:  J E Silbert; L S Freilich
Journal:  Biochem J       Date:  1980-08-15       Impact factor: 3.857

4.  Intracellular routing of GLcNAc-bearing molecules in thyrocytes: selective recycling through the Golgi apparatus.

Authors:  R Miquelis; J Courageot; A Jacq; O Blanck; C Perrin; P Bastiani
Journal:  J Cell Biol       Date:  1993-12       Impact factor: 10.539
  4 in total

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