W Nie1, Y Zhang, X Ru. 1. Beijing Institute of Pharmacology and Toxicology, Beijing 100850.
Abstract
OBJECTIVE: To conduct the activity evaluation for immunomodulating components from the Liuwei Dihuang Decoction (LWDHD). METHODS: Cyclophosphamide (Cy)-treated mice were used as the immunodeficient model and the antibody production response in plaque-forming cell (PFC) assay was employed as the activity-evaluating parameter. Stepwise fractionation was guided by activity-evaluation. RESULTS: Fr1, the ethanol-soluble fraction, and Fr2, the ethanol insoluble fraction of LWDHD, both significantly improved the antibody production response in Cy-treated mice, in which Fr2 showed stronger activity than that of Fr1. Fr2 was further fractionated. Fr2-CA4, one of the fractions obtained by active carbon chromatography from Fr2, exhibited the strongest activity in comparison with other fractions. CONCLUSION: CA4, which is mainly composed of acidic polysaccharides, is the main immunomodulating active fraction contained in the ethanol-insoluble fraction of LWDHD.
OBJECTIVE: To conduct the activity evaluation for immunomodulating components from the Liuwei Dihuang Decoction (LWDHD). METHODS:Cyclophosphamide (Cy)-treated mice were used as the immunodeficient model and the antibody production response in plaque-forming cell (PFC) assay was employed as the activity-evaluating parameter. Stepwise fractionation was guided by activity-evaluation. RESULTS:Fr1, the ethanol-soluble fraction, and Fr2, the ethanol insoluble fraction of LWDHD, both significantly improved the antibody production response in Cy-treated mice, in which Fr2 showed stronger activity than that of Fr1. Fr2 was further fractionated. Fr2-CA4, one of the fractions obtained by active carbon chromatography from Fr2, exhibited the strongest activity in comparison with other fractions. CONCLUSION:CA4, which is mainly composed of acidic polysaccharides, is the main immunomodulating active fraction contained in the ethanol-insoluble fraction of LWDHD.