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Literature DB >> 1147694

Thiamine pyrophosphatase and acid phosphatase. Their ultrastructural localization in rat skin.

Abstract

The ultrastructural localization of TPPase and AcPase in rat skin was studied. AcPase appeared localized in the lysosomes of the basal layer and in the keratinosomes of spinous, granulous and corneum layers. A diffuse reaction was also present in the granulous cells cytoplasm. Only the keratinosomes in the superficial layer were TPPase positive, specially those of the intercellular spaces of the proximal horny cells. The Golgi apparatus of all skin cells reacted strongly towards TPP. Membranous structures with TPPase and AcPase activity were evident within horny cells and seem to be infolded portions of the cell membrane which keep their enzymatic activity.

Entities: Chemical Species

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Year: 1975 PMID： 1147694

Source DB: PubMed Journal: Arch Dermatol Forsch ISSN： 0003-9187

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References

15 in total

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Journal: J Invest Dermatol Date: 1964-11 Impact factor: 8.551

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Authors: G F ODLAND
Journal: J Invest Dermatol Date: 1960-01 Impact factor: 8.551

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Authors: J Jonek; J Konecki; H Grzybek; Z Okkowski
Journal: Histochemie Date: 1971

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Authors: K Iijima
Journal: Acta Histochem Date: 1969 Impact factor: 2.479

Thiamine pyrophosphatase and acid phosphatase. Their ultrastructural localization in rat skin.

1. THE ULTRASTRUCTURAL LOCALIZATION OF ACID PHOSPHATASE IN HUMAN EPIDERMIS.

2. THE THIAMINE PYROPHOSPHATASE TECHNIQUE AS AN INDICATOR OF THE MORPHOLOGY OF THE GOLGI APPARATUS IN THE NEURONS. II. STUDIES ON THE CEREBRAL CORTEX.

3. A submicroscopic granular component in human epidermis.

4. Enzymatic hydrolysis of thiamine pyrophosphate by frozen sections of rat's kidney, adrenal and liver.

5. Electron microscopical studies on the lysosomes and the Golgi apparatus in the motoneurons of spinal cords of mice under physiological and pathological conditions.

6. Nucleoside diphosphatase and thiamine pyrophosphatase activities in the endoplasmic reticulum and golgi apparatus.

7. Cementsome, a new interpretation of the membrane-coating granule.

8. A fine-structure study of the human epidermal melanosome complex and its acid phosphatase activity.

9. Infolded plasma membranes of keratinized cells.

10. Histochemical studies on the morphology of the Golgi apparatus and on the relationship between the Golgi apparatus and lysosomes in the cellular elements of the rabbit area postrema with the thiamine pyrophosphatase and acid phosphatase methods.