Literature DB >> 11474029

Simplified microneutralization test for serotyping adenovirus isolates.

M D Malasig1, P R Goswami, L K Crawford-Miksza, D P Schnurr, G C Gray.   

Abstract

A simplified microneutralization procedure is described that uses an empirically determined virus challenge dose, a single dilution of antiserum, and observation of cytopathic effect to determine the adenovirus serotype. The simplified test has faster turnaround time and was 96% concordant with a confirmatory test using serial dilutions of type-specific sera. This method will find utility in high-volume serotyping work.

Mesh：

Year:  2001        PMID： 11474029      PMCID： PMC88276          DOI： 10.1128/JCM.39.8.2984-2986.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  Adult adenovirus infections: loss of orphaned vaccines precipitates military respiratory disease epidemics. For the Adenovirus Surveillance Group.

Authors:  G C Gray; P R Goswami; M D Malasig; A W Hawksworth; D H Trump; M A Ryan; D P Schnurr
Journal:  Clin Infect Dis       Date:  2000-10-04       Impact factor: 9.079

2.  Reemergence of adenovirus type 4 acute respiratory disease in military trainees: report of an outbreak during a lapse in vaccination.

Authors:  E M Barraza; S L Ludwig; J C Gaydos; J F Brundage
Journal:  J Infect Dis       Date:  1999-06       Impact factor: 5.226

3.  Quantitative colorimetric microneutralization assay for characterization of adenoviruses.

Authors:  L K Crawford-Miksza; D P Schnurr
Journal:  J Clin Microbiol       Date:  1994-09       Impact factor: 5.948

4.  Comparison of neutralization and DNA restriction enzyme methods for typing clinical isolates of human adenovirus.

Authors:  K H Fife; R Ashley; A F Shields; D Salter; J D Meyers; L Corey
Journal:  J Clin Microbiol       Date:  1985-07       Impact factor: 5.948

5.  Adenovirus vaccines in the U.S. military.

Authors:  C A Gaydos; J C Gaydos
Journal:  Mil Med       Date:  1995-06       Impact factor: 1.437

6.  Worldwide epidemiology of human adenovirus infections.

Authors:  H Schmitz; R Wigand; W Heinrich
Journal:  Am J Epidemiol       Date:  1983-04       Impact factor: 4.897

7.  Vero microcultures for adenovirus neutralization tests.

Authors:  J C Hierholzer; P G Bingham
Journal:  J Clin Microbiol       Date:  1978-06       Impact factor: 5.948
  7 in total
  13 in total

1.  Quantifying adenovirus-neutralizing antibodies by luciferase transgene detection: addressing preexisting immunity to vaccine and gene therapy vectors.

Authors:  Mieke C Sprangers; Wandena Lakhai; Wouter Koudstaal; Marielle Verhoeven; Björn F Koel; Ronald Vogels; Jaap Goudsmit; Menzo J E Havenga; Stefan Kostense
Journal:  J Clin Microbiol       Date:  2003-11       Impact factor: 5.948

2.  PCR analysis of egyptian respiratory adenovirus isolates, including identification of species, serotypes, and coinfections.

Authors:  David Metzgar; Miguel Osuna; Samuel Yingst; Magda Rakha; Kenneth Earhart; Diaa Elyan; Hala Esmat; Magdi D Saad; Adriana Kajon; Jianguo Wu; Gregory C Gray; Margaret A K Ryan; Kevin L Russell
Journal:  J Clin Microbiol       Date:  2005-11       Impact factor: 5.948

3.  Rapid detection and molecular serotyping of adenovirus by use of PCR followed by electrospray ionization mass spectrometry.

Authors:  Lawrence B Blyn; Thomas A Hall; Brian Libby; Raymond Ranken; Rangarajan Sampath; Karl Rudnick; Emily Moradi; Anjali Desai; David Metzgar; Kevin L Russell; Nikki E Freed; Melinda Balansay; Michael P Broderick; Miguel A Osuna; Steven A Hofstadler; David J Ecker
Journal:  J Clin Microbiol       Date:  2007-12-19       Impact factor: 5.948

4.  Outbreak of acute respiratory infection among infants in Lisbon, Portugal, caused by human adenovirus serotype 3 and a new 7/3 recombinant strain.

Authors:  Helena Rebelo-de-Andrade; Cristiana Pereira; Marta Gíria; Ema Prudêncio; Maria João Brito; Etelvina Calé; Nuno Taveira
Journal:  J Clin Microbiol       Date:  2010-02-10       Impact factor: 5.948

5.  Multiplexed Luminex xMAP assay for detection and identification of five adenovirus serotypes associated with epidemics of respiratory disease in adults.

Authors:  Cicely Washington; David Metzgar; Manzour Hernando Hazbón; Leonard Binn; Arthur Lyons; Carl Coward; Robert Kuschner
Journal:  J Clin Microbiol       Date:  2010-04-21       Impact factor: 5.948

6.  Molecular analysis of adenovirus isolates from vaccinated and unvaccinated young adults.

Authors:  Daniel A Blasiole; David Metzgar; Luke T Daum; Margaret A K Ryan; Jianguo Wu; Christopher Wills; Charles T Le; Nikki E Freed; Christian J Hansen; Gregory C Gray; Kevin L Russell
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948

7.  Evaluation of multiplex type-specific real-time PCR assays using the LightCycler and joint biological agent identification and diagnostic system platforms for detection and quantitation of adult human respiratory adenoviruses.

Authors:  David Metzgar; Carl Gibbins; N Ryan Hudson; Morris S Jones
Journal:  J Clin Microbiol       Date:  2010-01-06       Impact factor: 5.948

8.  Use of oligonucleotide microarrays for rapid detection and serotyping of acute respiratory disease-associated adenoviruses.

Authors:  Baochuan Lin; Gary J Vora; Dzung Thach; Elizabeth Walter; David Metzgar; Clark Tibbetts; David A Stenger
Journal:  J Clin Microbiol       Date:  2004-07       Impact factor: 5.948

9.  Vaccine-preventable adenoviral respiratory illness in US military recruits, 1999-2004.

Authors:  Kevin L Russell; Anthony W Hawksworth; Margaret A K Ryan; Jennifer Strickler; Marina Irvine; Christian J Hansen; Gregory C Gray; Joel C Gaydos
Journal:  Vaccine       Date:  2006-01-24       Impact factor: 3.641

10.  Human adenovirus 14a: a new epidemic threat.

Authors:  Gregory C Gray; Margaret L Chorazy
Journal:  J Infect Dis       Date:  2009-05-15       Impact factor: 5.226
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