Literature DB >> 11474024

Detection of antibodies to U.S. isolates of avian pneumovirus by a recombinant nucleocapsid protein-based sandwich enzyme-linked immunosorbent assay.

B R Gulati1, S Munir, D P Patnayak, S M Goyal, V Kapur.   

Abstract

The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the approximately 47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.

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Year:  2001        PMID： 11474024      PMCID： PMC88271          DOI： 10.1128/JCM.39.8.2967-2970.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

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2.  Isolation of avian pneumovirus from an outbreak of respiratory illness in Minnesota turkeys.

Authors:  S M Goyal; S J Chiang; A M Dar; K V Nagaraja; D P Shaw; D A Halvorson; V Kapur
Journal:  J Vet Diagn Invest       Date:  2000-03       Impact factor: 1.279

3.  Avian pneumovirus infection in Minnesota turkeys: experimental reproduction of the disease.

Authors:  F E Jirjis; S L Noll; D A Halvorson; K V Nagaraja; E L Townsend; A M Sheikh; D P Shaw
Journal:  Avian Dis       Date:  2000 Jan-Mar       Impact factor: 1.577

4.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

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Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  A modified enzyme-linked immunosorbent assay for the detection of avian pneumovirus antibodies.

Authors:  S Chiang; A M Dar; S M Goyal; M A Sheikh; J C Pedersen; B Panigrahy; D Senne; D A Halvorson; K V Nagaraja; V Kapur
Journal:  J Vet Diagn Invest       Date:  2000-07       Impact factor: 1.279

6.  Sequence analysis of the nucleocapsid and phosphoprotein genes of avian pneumoviruses circulating in the US.

Authors:  A M Dar; S Munir; S M Goyal; M S Abrahamsen; V Kapur
Journal:  Virus Res       Date:  2001-11-05       Impact factor: 3.303

7.  Analysis of the antibody response to bovine respiratory syncytial virus proteins in calves.

Authors:  F Westenbrink; T G Kimman; J M Brinkhof
Journal:  J Gen Virol       Date:  1989-03       Impact factor: 3.891

8.  Fusion protein predicted amino acid sequence of the first US avian pneumovirus isolate and lack of heterogeneity among other US isolates.

Authors:  B S Seal; H S Sellers; R J Meinersmann
Journal:  Virus Res       Date:  2000-02       Impact factor: 3.303

9.  Development of a highly sensitive and specific enzyme-linked immunosorbent assay based on recombinant matrix protein for detection of avian pneumovirus antibodies.

Authors:  B R Gulati; K T Cameron; B S Seal; S M Goyal; D A Halvorson; M K Njenga
Journal:  J Clin Microbiol       Date:  2000-11       Impact factor: 5.948

10.  Specific detection of avian pneumovirus (APV) US isolates by RT-PCR.

Authors:  H J Shin; G Rajashekara; F F Jirjis; D P Shaw; S M Goyal; D A Halvorson; K V Nagaraja
Journal:  Arch Virol       Date:  2000       Impact factor: 2.574
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Authors:  Marie-Eve Hamelin; Guy Boivin
Journal:  Clin Diagn Lab Immunol       Date:  2005-02

3.  Expression of recombinant small hydrophobic protein for serospecific detection of avian pneumovirus subgroup C.

Authors:  Lizhong Luo; Marta I Sabara; Yan Li
Journal:  Clin Diagn Lab Immunol       Date:  2005-01

4.  Development of a nucleoprotein-based enzyme-linked immunosorbent assay using a synthetic peptide antigen for detection of avian metapneumovirus antibodies in Turkey sera.

Authors:  Rene Alvarez; M Kariuki Njenga; Melissa Scott; Bruce S Seal
Journal:  Clin Diagn Lab Immunol       Date:  2004-03

5.  Development and evaluation of a blocking enzyme-linked immunosorbent assay for detection of avian metapneumovirus type C-specific antibodies in multiple domestic avian species.

Authors:  Elizabeth A Turpin; Dale C Lauer; David E Swayne
Journal:  J Clin Microbiol       Date:  2003-08       Impact factor: 5.948

6.  The pathogenicity of avian metapneumovirus subtype C wild bird isolates in domestic turkeys.

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  6 in total

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