Literature DB >> 11471730

Copper staining method for extracting biologically active proteins from native gels.

J Wen1, M Zhang, T P Horan, J S Philo, T Li, J Wypych, E A Mendiaz, K E Langley, K H Aoki, M Kuwamoto, Y Kita, T Arakawa.   

Abstract

An attempt was made to make protein bands visible on native gel using copper staining, since such a mild staining procedure would make the entire native gel electrophoresis process non-denaturing. Copper staining not only was able to detect various proteins on native gel with reasonable sensitivity, but also made extraction and recovery of active proteins possible from the gel using a gentle procedure.

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Year:  2001        PMID: 11471730     DOI: 10.1271/bbb.65.1315

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN: 0916-8451            Impact factor:   2.043


  1 in total

1.  Prothrombin and fibrinogen carbonylation: How that can affect the blood clotting.

Authors:  Hayk A Harutyunyan
Journal:  Redox Rep       Date:  2016-06-27       Impact factor: 4.412

  1 in total

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