Literature DB >> 11464917

PCR clamping.

H Orum1.   

Abstract

An efficient, PCR based method for the selective amplification of DNA target sequences that differs by a single base pair is described. The method utilises the high affinity and specificity of PNA for their complementary nucleic acids and that PNA cannot function as primers for DNA polymerases.

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Year:  2000        PMID： 11464917

Source DB:  PubMed          Journal:  Curr Issues Mol Biol        ISSN： 1467-3037            Impact factor:   2.081


  11 in total

1.  One-step detection of c-kit point mutations using peptide nucleic acid-mediated polymerase chain reaction clamping and hybridization probes.

Authors:  Karl Sotlar; Luis Escribano; Olfert Landt; Stefanie Möhrle; Sonia Herrero; Antonio Torrelo; Ulrich Lass; Hans-Peter Horny; Burkhard Bültmann
Journal:  Am J Pathol       Date:  2003-03       Impact factor: 4.307

2.  Investigation on natural diets of larval marine animals using peptide nucleic acid-directed polymerase chain reaction clamping.

Authors:  Seinen Chow; Sayaka Suzuki; Tadashi Matsunaga; Shane Lavery; Andrew Jeffs; Haruko Takeyama
Journal:  Mar Biotechnol (NY)       Date:  2010-06-10       Impact factor: 3.619

3.  The use of COLD-PCR and high-resolution melting analysis improves the limit of detection of KRAS and BRAF mutations in colorectal cancer.

Authors:  Irene Mancini; Claudio Santucci; Roberta Sestini; Lisa Simi; Nicola Pratesi; Fabio Cianchi; Rosa Valanzano; Pamela Pinzani; Claudio Orlando
Journal:  J Mol Diagn       Date:  2010-07-08       Impact factor: 5.568

4.  Coamplification at lower denaturation temperature-PCR increases mutation-detection selectivity of TaqMan-based real-time PCR.

Authors:  Jin Li; Lilin Wang; Pasi A Jänne; G Mike Makrigiorgos
Journal:  Clin Chem       Date:  2009-02-20       Impact factor: 8.327

5.  Systemic mastocytosis associated with chronic idiopathic myelofibrosis: a distinct subtype of systemic mastocytosis associated with a [corrected] clonal hematological non-mast [corrected] cell lineage disorder carrying the activating point mutations KITD816V and JAK2V617F.

Authors:  Karl Sotlar; Anja Bache; Florian Stellmacher; Burkhard Bültmann; Peter Valent; Hans-Peter Horny
Journal:  J Mol Diagn       Date:  2007-12-28       Impact factor: 5.568

6.  A Novel, Highly Selective RT-QPCR Method for Quantification of MSRV Using PNA Clamping Syncytin-1 (ERVWE1).

Authors:  Grzegorz Machnik; Estera Skudrzyk; Łukasz Bułdak; Krzysztof Łabuzek; Jarosław Ruczyński; Magdalena Alenowicz; Piotr Rekowski; Piotr Jan Nowak; Bogusław Okopień
Journal:  Mol Biotechnol       Date:  2015-09       Impact factor: 2.695

7.  Application of nonsense-mediated primer exclusion (NOPE) for preparation of unique molecular barcoded libraries.

Authors:  Dmitriy A Shagin; Maria A Turchaninova; Irina A Shagina; Mikhail Shugay; Andrew R Zaretsky; Olga I Zueva; Dmitriy A Bolotin; Sergey Lukyanov; Dmitriy M Chudakov
Journal:  BMC Genomics       Date:  2017-06-05       Impact factor: 3.969

Review 8.  Fluorogenic PNA probes.

Authors:  Tirayut Vilaivan
Journal:  Beilstein J Org Chem       Date:  2018-01-29       Impact factor: 2.883

Review 9.  Current Update of Laboratory Molecular Diagnostics Advancement in Management of Colorectal Cancer (CRC).

Authors:  Siew-Wai Pang; Noel Jacques Awi; Subasri Armon; Wendy Wan-Dee Lim; John Seng-Hooi Low; Kaik-Boo Peh; Suat-Cheng Peh; Sin-Yeang Teow
Journal:  Diagnostics (Basel)       Date:  2019-12-23

Review 10.  Porcine reproductive and respiratory syndrome virus genetic variability a management and diagnostic dilemma.

Authors:  Jessica Risser; Matthew Ackerman; Robert Evelsizer; Stephen Wu; Byungjoon Kwon; James Mark Hammer
Journal:  Virol J       Date:  2021-10-18       Impact factor: 4.099
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