[Studies on the culture of clostridium butyricum strain M 55 ATCC 13.732. 4th Communication: The influence of peptone quality upon kininase activity (author's transl)].

Studies of the oncolytic effect of Cl. butyricum strain M 55 resulted in the hypothesis of oncolysis to be an effect of a continuous production of kinin-decomposing enzymes. A decomposition of bradykinin will reduce capillary permeability and microcirculation in the tumour tissue, thus releasing and maintaining the process of necrotization. To achieve an optimal oncolytic effect of Cl. butyricum M 55, in addition to conditions already demanded in preceding papers, also an abundant production of kininase is required. Experience has shown peptone-containing media to be most suitable for culturing the M 55 strain, kininase formation being dependent upon peptone quality. The best yield of kinine-decomposing enzymes was achieved with the following peptones: peptone from Gelatine Merck, Tryptose Merck, Microbiotone Oxoid, Proteose Oxoid and Trypticase BBL. To obtain the greatest possible kininase production by the spores of Cl. butyricum M 55 applied for oncolytic therapy, the peptones mentioned are recommended for culture.