The modification of NMDA receptors by visual experience in the rat retina is age dependent.

Extensive studies have shown that the activation of N-methyl-D-aspartate receptors (NMDARs) and the subsequent rise in the levels of postsynaptic calcium are critical events in the initiation of synaptic plasticity. Modification of the amount, or of the subunit composition of NMDARs, alters receptor function thereby affecting the development and/or efficacy of synaptic transmission. In the present study, a Western blot analysis was employed to investigate the effects of visual experience and age on the differential expression of NMDARs in the rat retina. A crude synaptic membrane fraction (SPM) was prepared and assayed with antibodies specific for either the NR1, NR2A or NR2B subunits. Relative to control animals raised in a diurnal light-dark cycle, a period of 1 week of dark-rearing caused an increase in the relative amount of NR1, a decrease in the level of NR2A, and no change in the level of NR2B subunit expression in postnatal day 12 rats. At 2 months of age, 1 week of dark-rearing had less effect, and at 6 months of age there was no difference between dark-reared and control animals. The effect of light exposure on dark-reared animals was tested for the 2-month-old animals. Light exposure for long periods (days), but not short periods (h), could reverse the dark-rearing effects. These data provide evidence for a developmentally regulated plasticity of NMDAR subunits in the retina.