The occurrence of hepatitis A and astroviruses in selected river and dam waters in South Africa.

Over a period of one year (June 1997-May 1998) samples of surface waters, used for domestic and recreational purposes, were collected weekly from the same sites on the Klip River and Vaal Dam, Gauteng, South Africa. Sensitive and specific reverse transcriptase-polymerase chain reaction-oligonucleotide probe assays were used to detect HAV and HAstV RNA in concentrates of the water and infectious virus in cell cultures infected with the water concentrates. HAV was detected in 18 (35.3%) of the river and 19 (37.3%) of the dam water samples, often in association with the RNA from other enteric viruses. HAstV was detected less frequently and was present in 11 (21.6%) of the river and 3 (5.9%) of the dam water samples. A seasonal pattern was noted for HAV but not for HAstV. Cell culture amplification in a variety of carefully selected cell culture systems enhanced the detection of both viruses. Infectious viruses were detected in dam water samples where microbiological indicators of faecal pollution were absent or within acceptable limits. The presence of these viruses in the dam and river water could pose a potential health risk for people using these waters for domestic or recreational purposes.