OBJECTIVE: The present study was designed to analyse the relationship between apoptosis related proteins (bcl-2 and bax), tumour suppressor protein p53, proliferation markers (PCNA and mitotic index), human papillomavirus (HPV) and angiogenesis in cervical cancer and their impact on clinical outcome. STUDY DESIGN: Tumours from 111 patients were assessed by immunohistochemistry for the expression of bcl-2, bax, p53 and PCNA, by PCR for the presence of HPV-DNA, for the quantification of the mitotic index and the microvessel density (CD 31). The results were correlated with various histopathologic characteristics and survival. RESULTS: The multiple Cox's regression analysis for overall survival of all prognostic variables gave as best model: bcl-2 (P<0.001), lymphovascular permeation (P=0.004), mitotic index (P=0.019), tumour grade (P=0.048) and FIGO stage (P=0.070). Subanalysis was performed for the patients where the lymph node status was known (n=79). Adding the lymph node status gave as best model for overall survival bcl-2 (P=0.001), lymphovascular permeation (P=0.003) and mitotic index (P=0.044). However, they hardly influenced the association. CONCLUSION: In the apoptotic pathway of cervical cancer, bcl-2 is one of most important proteins. It can probably not only mediate cell death but also regulate cell growth. A better understanding of their relations will probably provide the basis for more rational cancer therapies in the future.
OBJECTIVE: The present study was designed to analyse the relationship between apoptosis related proteins (bcl-2 and bax), tumour suppressor protein p53, proliferation markers (PCNA and mitotic index), human papillomavirus (HPV) and angiogenesis in cervical cancer and their impact on clinical outcome. STUDY DESIGN: Tumours from 111 patients were assessed by immunohistochemistry for the expression of bcl-2, bax, p53 and PCNA, by PCR for the presence of HPV-DNA, for the quantification of the mitotic index and the microvessel density (CD 31). The results were correlated with various histopathologic characteristics and survival. RESULTS: The multiple Cox's regression analysis for overall survival of all prognostic variables gave as best model: bcl-2 (P<0.001), lymphovascular permeation (P=0.004), mitotic index (P=0.019), tumour grade (P=0.048) and FIGO stage (P=0.070). Subanalysis was performed for the patients where the lymph node status was known (n=79). Adding the lymph node status gave as best model for overall survival bcl-2 (P=0.001), lymphovascular permeation (P=0.003) and mitotic index (P=0.044). However, they hardly influenced the association. CONCLUSION: In the apoptotic pathway of cervical cancer, bcl-2 is one of most important proteins. It can probably not only mediate cell death but also regulate cell growth. A better understanding of their relations will probably provide the basis for more rational cancer therapies in the future.
Authors: Mariana Gamba De Paula Eduardo; Adriana Bittencourt Campaner; Maria Antonieta Longo Galvão Silva Journal: Pathol Oncol Res Date: 2015-02-15 Impact factor: 3.201
Authors: Z Protrka; S Arsenijevic; A Dimitrijevic; S Mitrovic; V Stankovic; M Milosavljevic; T Kastratovic; J Djuric Journal: Eur J Histochem Date: 2011-03-16 Impact factor: 3.188
Authors: Gorka Lasso; Sandra V Mayer; Evandro R Winkelmann; Tim Chu; Oliver Elliot; Juan Angel Patino-Galindo; Kernyu Park; Raul Rabadan; Barry Honig; Sagi D Shapira Journal: Cell Date: 2019-08-29 Impact factor: 41.582
Authors: Sergio M Amaro-Filho; Jonathan E Golub; Gerard J Nuovo; Cynthia B Cunha; José E Levi; Luisa L Villa; Cecília V Andrade; Fabio B Russomano; Aparecida Tristão; Andrea Pires; Alcina F Nicol Journal: PLoS One Date: 2013-03-07 Impact factor: 3.240