| Literature DB >> 11448885 |
R M Podowski1, E L Sonnhammer.
Abstract
UNLABELLED: MEDUSA is a tool for automatic selection and visual assessment of PCR primer pairs, developed to assist large scale gene expression analysis projects. The system allows specification of constraints of the location and distances between the primers in a pair. For instance, primers in coding, non-coding, exon/intron-spanning regions might be selected. Medusa applies these constraints as a filter to primers predicted by three external programs, and displays the resulting primer pairs graphically in the Blixem (Sonnhammer and Durbin, COMPUT: Appl. Biosci. 10, 301-307, 1994; http://www.cgr.ki.se/cgr/groups/sonnhammer/Blixem.html) viewer. AVAILABILITY: The MEDUSA web server is available at http://www.cgr.ki.se/cgr/MEDUSA. The source code and user information are available at ftp://ftp.cgr.ki.se/pub/prog/medusa.Mesh：
Substances：
Year: 2001 PMID： 11448885 DOI： 10.1093/bioinformatics/17.7.656
Source DB: PubMed Journal: Bioinformatics ISSN： 1367-4803 Impact factor: 6.937