PURPOSE: To define the physiological and structural changes that may accompany aging in the normal mouse retina. METHODS: C57BL/6 mice were maintained under cyclic light for either 2, 6, or 12 months. After rod- and cone-mediated corneal electroretinograms (ERG's) were recorded from anesthetized animals, the retinal structure was quantitatively examined. Photoreceptor cell density was measured within 100-microm regions of the central superior and inferior retina. Cone photoreceptor subtypes were identified by immunocytochemistry. RESULTS: The amplitudes of rod- and cone-mediated ERG's were reduced in older mice, although the overall ERG wave-form did not change appreciably and implicit times were not changed in an age-dependent fashion. In comparison, there was no significant age-related decline in rod or cone photoreceptor density. CONCLUSIONS: The amplitude of the mouse ERG declines with age. This change does not appear to reflect a change in the structural integrity of the photoreceptor cells. In functional studies of murine models of late-onset retinal disorders, it will be important to take these changes into consideration.
PURPOSE: To define the physiological and structural changes that may accompany aging in the normal mouse retina. METHODS: C57BL/6 mice were maintained under cyclic light for either 2, 6, or 12 months. After rod- and cone-mediated corneal electroretinograms (ERG's) were recorded from anesthetized animals, the retinal structure was quantitatively examined. Photoreceptor cell density was measured within 100-microm regions of the central superior and inferior retina. Cone photoreceptor subtypes were identified by immunocytochemistry. RESULTS: The amplitudes of rod- and cone-mediated ERG's were reduced in older mice, although the overall ERG wave-form did not change appreciably and implicit times were not changed in an age-dependent fashion. In comparison, there was no significant age-related decline in rod or cone photoreceptor density. CONCLUSIONS: The amplitude of the mouseERG declines with age. This change does not appear to reflect a change in the structural integrity of the photoreceptor cells. In functional studies of murine models of late-onset retinal disorders, it will be important to take these changes into consideration.
Authors: Kenkichi Baba; Ilaria Piano; Polina Lyuboslavsky; Micah A Chrenek; Jana T Sellers; Shuo Zhang; Claudia Gargini; Li He; Gianluca Tosini; P Michael Iuvone Journal: Proc Natl Acad Sci U S A Date: 2018-11-29 Impact factor: 11.205
Authors: M Joseph Phillips; Sarah Webb-Wood; Amanda E Faulkner; Seema B Jabbar; Valerie Biousse; Nancy J Newman; Vi T Do; Jeffrey H Boatright; Douglas C Wallace; Machelle T Pardue Journal: Invest Ophthalmol Vis Sci Date: 2010-07-29 Impact factor: 4.799
Authors: Nicholas J Reish; Astha Maltare; Alex S McKeown; Ann M Laszczyk; Timothy W Kraft; Alecia K Gross; Gwendalyn D King Journal: Invest Ophthalmol Vis Sci Date: 2013-10-11 Impact factor: 4.799
Authors: Sharee Kuny; Frédéric Gaillard; Silvina C Mema; Paul R Freund; Kang Zhang; Ian M Macdonald; Janet R Sparrow; Yves Sauvé Journal: Invest Ophthalmol Vis Sci Date: 2009-11-20 Impact factor: 4.799