Literature DB >> 11443276

Structural studies of the glycopeptides of B-chain of cinnamomin--a type II ribosome-inactivating protein by nuclear magnetic resonance.

Z Pu1, Y Li, F J Hou, F He, N Zhang, H Wu, W Y Liu.   

Abstract

Cinnamomin is a plant type II ribosome-inactivating protein (RIP) isolated from the seeds of Cinnamomum camphora. It consists of two nonidentical polypeptide chains (A- and B-chain) held together through one disulfide linkage. Its A- and B-chain contain 0.3% and 3.9% sugars respectively. The B-chain of cinnamomin was digested by pronase E and then the liberated glycopeptides were separated from non-glycopeptides by gel filtration chromatography on a Bio-Gel P-4 column. Three crude glycopeptides were obtained by continuing chromatography over anion-exchange resin (AG1-X2) in the buffer of 2% pyridine-acetic acid (pH 8.3) with a polygradient elution system. Through further purification by the gel filtration chromatography and HPLC, three major glycopeptides, GP1, GP2 and GP3 were obtained. Mainly by two-dimensional Nuclear Magnetic Resonance (NMR) including TOCSY, DQF-COSY, NOESY, HMQC and HMBC, their primary structures were analyzed as: Man\balpha1,3Man\balpha1,6(Man\balpha1,3)(Xyl\bbeta1,2)Man\bbeta1,4GlcNAc\bbeta1,4GlcNAc\bbeta1-(Gly-)Asn-Asn-Thr(GP1), Man\balpha1,6(Man\balpha1,3)(Xyl\bbeta1,2)Man\bbeta1,4GlcNAc\bbeta1,4(Fuc\balpha1,3)GlcNAc\bbeta1-Asn-Ala-Thr(GP2),Man\balpha1,6(Man\balpha1,3)Man\balpha1,6(Man\balpha 1,2 Man\balpha1,3)Man\bbeta1,4GlcNAc\bbeta1,4GlcNAc\bbeta1-(Ala-)Asn-Gly-Thr(GP3).

Entities:  

Mesh：

Substances：

Year:  2000        PMID： 11443276     DOI： 10.1023/a:1010957007038

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  15 in total

Review 1.  NMR spectroscopy in the structural elucidation of oligosaccharides and glycosides.

Authors:  P K Agrawal
Journal:  Phytochemistry       Date:  1992-10       Impact factor: 4.072

2.  Simultaneous existence of two types of ribosome-inactivating proteins in the seeds of Cinnamonum camphora--characterization of the enzymatic activities of these cytotoxic proteins.

Authors:  J Ling; W Y Liu; T P Wang
Journal:  Biochim Biophys Acta       Date:  1995-09-27

3.  Substrate Specificities of N-Acetylglucosaminyl-, Fucosyl-, and Xylosyltransferases that Modify Glycoproteins in the Golgi Apparatus of Bean Cotyledons.

Authors:  K D Johnson; M J Chrispeels
Journal:  Plant Physiol       Date:  1987-08       Impact factor: 8.340

Review 4.  Ribosome-inactivating proteins from plants.

Authors:  L Barbieri; M G Battelli; F Stirpe
Journal:  Biochim Biophys Acta       Date:  1993-12-21

5.  Structural analysis of N-glycans of storage glycoproteins in soybean (Glycine max. L) seed.

Authors:  Y Kimura; A Ohno; S Takagi
Journal:  Biosci Biotechnol Biochem       Date:  1997-11       Impact factor: 2.043

6.  Conformation and function of the N-linked glycan in the adhesion domain of human CD2.

Authors:  D F Wyss; J S Choi; J Li; M H Knoppers; K J Willis; A R Arulanandam; A Smolyar; E L Reinherz; G Wagner
Journal:  Science       Date:  1995-09-01       Impact factor: 47.728

7.  Characterization of the carbohydrate moiety of Clerodendron trichotomum lectins. Its structure and reactivity toward plant lectins.

Authors:  H Kitagaki-Ogawa; I Matsumoto; N Seno; N Takahashi; S Endo; Y Arata
Journal:  Eur J Biochem       Date:  1986-12-15

8.  Structures of sugar chains of ricin D.

Authors:  Y Kimura; S Hase; Y Kobayashi; Y Kyogoku; T Ikenaka; G Funatsu
Journal:  J Biochem       Date:  1988-06       Impact factor: 3.387

9.  The nature of the carbohydrate-peptide linkage region in glycoproteins from the cellulosomes of Clostridium thermocellum and Bacteroides cellulosolvens.

Authors:  G J Gerwig; J P Kamerling; J F Vliegenthart; E Morag; R Lamed; E A Bayer
Journal:  J Biol Chem       Date:  1993-12-25       Impact factor: 5.157

10.  Primary structures of N-linked oligosaccharides of momordin-a, a ribosome-inactivating protein from Momordica charantia seeds.

Authors:  Y Kimura; Y Minami; T Tokuda; S Nakajima; S Takagi; G Funatsu
Journal:  Agric Biol Chem       Date:  1991-08
View more
  3 in total

1.  Research on ribosome-inactivating proteins from angiospermae to gymnospermae and cryptogamia.

Authors:  Wang-Yi Liu
Journal:  Am J Transl Res       Date:  2017-12-15       Impact factor: 4.060

2.  Structural analysis of N- and O-glycans using ZIC-HILIC/dialysis coupled to NMR detection.

Authors:  Yi Qu; Ju Feng; Shuang Deng; Li Cao; Qibin Zhang; Rui Zhao; Zhaorui Zhang; Yuxuan Jiang; Erika M Zink; Scott E Baker; Mary S Lipton; Ljiljana Paša-Tolić; Jian Zhi Hu; Si Wu
Journal:  Fungal Genet Biol       Date:  2014-08-10       Impact factor: 3.495

3.  Cinnamomin, a type II ribosome-inactivating protein, is a storage protein in the seed of the camphor tree (Cinnamomum camphora).

Authors:  Ren-shui Liu; Guo-qing Wei; Qiang Yang; Wen-jun He; Wang-Yi Liu
Journal:  Biochem J       Date:  2002-03-15       Impact factor: 3.857
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.