A comparative study of propolis and saline application on human dentin. A SEM study.

The purpose of this study was to compare the texture changes of dentinal surfaces after the application of propolis and saline. Twenty-four recently extracted human premolar teeth were obtained from the Division of Oral and Maxillofacial Surgery, King Saud University, College of Dentistry, Riyadh. The teeth were classified as sound, periodontally involved and with recession. Forty-eight, 3 x 3 mm dentin disc specimens were prepared and only 24 specimens were treated with propolis (pH 8.5) and saline (pH 6). They were treated in 4 categories i.e.: (1) no application of any material (control); (2) propolis for 60 seconds; (3) propolis for 120 seconds and (4) saline for 60 seconds. All experimental specimens were burnished with cotton pellet with propolis and saline for the prescribed time. All specimens were prepared for scanning electron microscopy (SEM) with sputter technique, (Blomlof and Lindskog, 1995), and examined in the SEM (Jeol, Japan) operated at 25 KV with a tilt angle between 0-30 degrees. The bar was 10 mm and magnification 2000x. There were dentinal tubules occlusion in all three groups of dentin treated with propolis for 120 seconds. The propolis was better than saline in occluding dentinal tubules. It may be suggested that further research is needed for double blind clinical trials of propolis in patients with dentinal hypersensitivity.