N Ostendorf1, D Niefhoff, U Cassens, W Sibrowski. 1. Department of Transfusion Medicine and Transplantation Immunology, Westfaelische Wilhelms-Universitaet, Münster, Germany. transfusionsmedizin@uni-muenster.de
Abstract
BACKGROUND AND OBJECTIVES: To prove the feasibility of a semi-automated cross-match procedure using a commercially available solid-phase microplate test and standard laboratory equipment. MATERIAL AND METHODS: The new procedure was evaluated against the conventional spin tube technique and the gel centrifugation system. RESULTS: The sensitivity of the method and the rate of non-specific reactions were equal to those for the other test systems. The samples taken from the red cell concentrates for cross-matching remained stable for the shelf-life of the product. CONCLUSION: The semi-automated cross-match was successfully introduced in our routine laboratory as a means to process large numbers of tests.
BACKGROUND AND OBJECTIVES: To prove the feasibility of a semi-automated cross-match procedure using a commercially available solid-phase microplate test and standard laboratory equipment. MATERIAL AND METHODS: The new procedure was evaluated against the conventional spin tube technique and the gel centrifugation system. RESULTS: The sensitivity of the method and the rate of non-specific reactions were equal to those for the other test systems. The samples taken from the red cell concentrates for cross-matching remained stable for the shelf-life of the product. CONCLUSION: The semi-automated cross-match was successfully introduced in our routine laboratory as a means to process large numbers of tests.
Authors: Rozieyati Mohamed Saleh; Zulkafli Zefarina; Nor Fazila Che Mat; Geoffrey Keith Chambers; Hisham Atan Edinur Journal: Int J Prev Med Date: 2018-05-16