I Mader-Wołyńska1, D Zwolińska. 1. Katedra i Klinika Nefrologii Pediatrycznej Akademii Medycznej we Wrocławiu.
Abstract
UNLABELLED: The aim of the study was to estimate antioxidant status and reliable factors involved in antioxidant protection in children with nephrotic syndrome (NS). 37 children (20 boys, 17 girls) with nephrotic syndrome (NS), aged 2.5-17 (mean 9.5), were included into the study. Erythrocyte Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), reduced glutathione (GSH) and malonylodialdehide (MDA) was estimated using commercial kits produced by Calbiochem and Bioxytech. RESULTS: 1) Decreased mean values of following parameters in children with NS--acute phase comparing to remission phase and the control group: GSH-Px (123.88 +/- 25.02; 187.69 +/- 24.57; 266.49 +/- 21.84 mU/ml; p < 0.001), GSH: (16.6 +/- 5.99; 27.81 +/- 7.68; 43.79 +/- 7.21 mumol/L; p < 0.001). 2) Increased mean values of following parameters in adequate groups: MDA: (18.70 +/- 2.01; 14.63 +/- 1.51; 9.53 +/- 1.48 mumol, p < 0.001), SOD: 536.27 +/- 450.49; 387.16 +/- 219.49; 236.96 +/- 85.71 U/mg). IN CONCLUSION: there is increased lipid peroxidation and insufficient antioxidant defence in children with nephrotic syndrome.
UNLABELLED: The aim of the study was to estimate antioxidant status and reliable factors involved in antioxidant protection in children with nephrotic syndrome (NS). 37 children (20 boys, 17 girls) with nephrotic syndrome (NS), aged 2.5-17 (mean 9.5), were included into the study. Erythrocyte Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), reduced glutathione (GSH) and malonylodialdehide (MDA) was estimated using commercial kits produced by Calbiochem and Bioxytech. RESULTS: 1) Decreased mean values of following parameters in children with NS--acute phase comparing to remission phase and the control group: GSH-Px (123.88 +/- 25.02; 187.69 +/- 24.57; 266.49 +/- 21.84 mU/ml; p < 0.001), GSH: (16.6 +/- 5.99; 27.81 +/- 7.68; 43.79 +/- 7.21 mumol/L; p < 0.001). 2) Increased mean values of following parameters in adequate groups: MDA: (18.70 +/- 2.01; 14.63 +/- 1.51; 9.53 +/- 1.48 mumol, p < 0.001), SOD: 536.27 +/- 450.49; 387.16 +/- 219.49; 236.96 +/- 85.71 U/mg). IN CONCLUSION: there is increased lipid peroxidation and insufficient antioxidant defence in children with nephrotic syndrome.