Literature DB >> 11431124

Voltage-clamp recordings of postsynaptic currents in substantia gelatinosa neurons in vitro and its applications to assess synaptic transmission.

K Yang1, Y Li, E Kumamoto, H Furue, M Yoshimura.   

Abstract

We describe here procedures for recording postsynaptic currents in substantia gelatinosa neurons on a transverse spinal cord slice preparation with an attached dorsal root. At the holding potential of -70 mV, glutamatergic spontaneous excitatory postsynaptic currents (EPSCs) and dorsal root (A delta and/or C fiber) stimulation-evoked EPSCs could be observed. Whereas at the holding potential of 0 mV, spontaneous inhibitory postsynaptic currents (IPSCs) and dorsal root A delta fiber stimulation-evoked IPSCs could be encountered. The methods make it possible to evaluate synaptic transmission by analysing the postsynaptic currents on dorsal root attached spinal cord slice.

Mesh：

Year:  2001        PMID： 11431124     DOI： 10.1016/s1385-299x(01)00069-1

Source DB:  PubMed          Journal:  Brain Res Brain Res Protoc        ISSN： 1385-299X


  14 in total

1.  Electrophysiological mapping of the nociceptive inputs to the substantia gelatinosa in rat horizontal spinal cord slices.

Authors:  Go Kato; Hidemasa Furue; Toshihiko Katafuchi; Toshiharu Yasaka; Yukihide Iwamoto; Megumu Yoshimura
Journal:  J Physiol       Date:  2004-08-05       Impact factor: 5.182

2.  Activation of group I mGlu receptors contributes to facilitation of NMDA receptor membrane current in spinal dorsal horn neurons after hind paw inflammation in rats.

Authors:  Kun Yang; Keita Takeuchi; Feng Wei; Ronald Dubner; Ke Ren
Journal:  Eur J Pharmacol       Date:  2011-09-21       Impact factor: 4.432

3.  Olfactory bulb external tufted cells are synchronized by multiple intraglomerular mechanisms.

Authors:  Abdallah Hayar; Michael T Shipley; Matthew Ennis
Journal:  J Neurosci       Date:  2005-09-07       Impact factor: 6.167

4.  Cell-type-specific excitatory and inhibitory circuits involving primary afferents in the substantia gelatinosa of the rat spinal dorsal horn in vitro.

Authors:  Toshiharu Yasaka; Go Kato; Hidemasa Furue; Md Harunor Rashid; Motoki Sonohata; Akihiro Tamae; Yuzo Murata; Sadahiko Masuko; Megumu Yoshimura
Journal:  J Physiol       Date:  2007-03-08       Impact factor: 5.182

5.  Spinal cord mechanisms mediating behavioral hyperalgesia induced by neurokinin-1 tachykinin receptor activation in the rostral ventromedial medulla.

Authors:  S C Lagraize; W Guo; K Yang; F Wei; K Ren; R Dubner
Journal:  Neuroscience       Date:  2010-10-01       Impact factor: 3.590

6.  TRPA1-expressing primary afferents synapse with a morphologically identified subclass of substantia gelatinosa neurons in the adult rat spinal cord.

Authors:  Daisuke Uta; Hidemasa Furue; Anthony E Pickering; Md Harunor Rashid; Hiroko Mizuguchi-Takase; Toshihiko Katafuchi; Keiji Imoto; Megumu Yoshimura
Journal:  Eur J Neurosci       Date:  2010-05-24       Impact factor: 3.386

7.  Postnatal Excitability Development and Innervation by Functional Transient Receptor Potential Vanilloid 1 (TRPV1) Terminals in Neurons of the Rat Spinal Sacral Dorsal Commissural Nucleus: an Electrophysiological Study.

Authors:  Kun Yang
Journal:  Mol Neurobiol       Date:  2015-11-02       Impact factor: 5.590

8.  TRP Channels Involved in Spontaneous L-Glutamate Release Enhancement in the Adult Rat Spinal Substantia Gelatinosa.

Authors:  Eiichi Kumamoto; Tsugumi Fujita; Chang-Yu Jiang
Journal:  Cells       Date:  2014-04-29       Impact factor: 6.600

Review 9.  Differential Activation of TRP Channels in the Adult Rat Spinal Substantia Gelatinosa by Stereoisomers of Plant-Derived Chemicals.

Authors:  Eiichi Kumamoto; Tsugumi Fujita
Journal:  Pharmaceuticals (Basel)       Date:  2016-07-28

10.  CCL-1 in the spinal cord contributes to neuropathic pain induced by nerve injury.

Authors:  N Akimoto; K Honda; D Uta; K Beppu; Y Ushijima; Y Matsuzaki; S Nakashima; M A Kido; K Imoto; Y Takano; M Noda
Journal:  Cell Death Dis       Date:  2013-06-20       Impact factor: 8.469
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.