Literature DB >> 11430409

PCR cloning of type II polyhydroxyalkanoate biosynthesis genes from two Pseudomonas strains.

G Zhang1, X Hang, P Green, K P Ho, G Q Chen.   

Abstract

Two polyhydroxyalkanoate synthase genes, phaC1 from Pseudomonas pseudoalcaligenes HBQ06 and phaC2 from Pseudomonas nitroreducens 0802, were cloned using a PCR cloning strategy based on the type II pha loci property of Pseudomonas strains. The complete open reading frames (ORFs) of phaC1 (P. nitroreducens HBQ06) and phaC2 (P. nitroreducens 0802) were identified from the PCR products. Using the sequence information, the complete PHA synthase genes were PCR cloned directly from the genomic DNA and expressed in Escherichia coli as confirmed by Fourier transform-infrared spectroscopy and gas chromatography. The differences between PhaC1 and PhaC2 were analyzed and the two proteins were suggested to contain different functions and evolution history.

Entities:  

Mesh：

Substances：

Year:  2001        PMID： 11430409     DOI： 10.1111/j.1574-6968.2001.tb10637.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  8 in total

Review 1.  The TetR family of transcriptional repressors.

Authors:  Juan L Ramos; Manuel Martínez-Bueno; Antonio J Molina-Henares; Wilson Terán; Kazuya Watanabe; Xiaodong Zhang; María Trinidad Gallegos; Richard Brennan; Raquel Tobes
Journal:  Microbiol Mol Biol Rev       Date:  2005-06       Impact factor: 11.056

2.  Inactivation of type I polyhydroxyalkanoate synthase in Aeromonas hydrophila resulted in discovery of another potential PHA synthase.

Authors:  Fengqing Hu; Song You
Journal:  J Ind Microbiol Biotechnol       Date:  2006-10-17       Impact factor: 3.346

3.  The influence of nitrogen limitation on mcl-PHA synthesis by two newly isolated strains of Pseudomonas sp.

Authors:  Slawomir Ciesielski; Justyna Mozejko; Grzegorz Przybyłek
Journal:  J Ind Microbiol Biotechnol       Date:  2010-03-04       Impact factor: 3.346

4.  Cloning, Sequencing and In Silico Analysis of phbC Gene from Pseudomonas spp.

Authors:  Mukesh R Jangra; Ritu Batra; Nishat Passricha; Virendra K Sikka
Journal:  Indian J Microbiol       Date:  2018-11-12       Impact factor: 2.461

5.  Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains.

Authors:  Philipp Höfer; Young J Choi; Michael J Osborne; Carlos B Miguez; Patrick Vermette; Denis Groleau
Journal:  Microb Cell Fact       Date:  2010-09-16       Impact factor: 5.328

6.  Synthesis of Diblock copolymer poly-3-hydroxybutyrate -block-poly-3-hydroxyhexanoate [PHB-b-PHHx] by a β-oxidation weakened Pseudomonas putida KT2442.

Authors:  Lakshmi Tripathi; Lin-Ping Wu; Jinchun Chen; Guo-Qiang Chen
Journal:  Microb Cell Fact       Date:  2012-04-05       Impact factor: 5.328

7.  Whole genome amplification approach reveals novel polyhydroxyalkanoate synthases (PhaCs) from Japan Trench and Nankai Trough seawater.

Authors:  Choon Pin Foong; Nyok-Sean Lau; Shigeru Deguchi; Takashi Toyofuku; Todd D Taylor; Kumar Sudesh; Minami Matsui
Journal:  BMC Microbiol       Date:  2014-12-24       Impact factor: 3.605

8.  Screening of polyhydroxyalkanoate-producing bacteria and PhaC-encoding genes in two hypersaline microbial mats from Guerrero Negro, Baja California Sur, Mexico.

Authors:  Carolina A Martínez-Gutiérrez; Hever Latisnere-Barragán; José Q García-Maldonado; Alejandro López-Cortés
Journal:  PeerJ       Date:  2018-05-07       Impact factor: 2.984
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.