Literature DB >> 11425761

Exo-arabinanase of Penicillium chrysogenum able to release arabinobiose from alpha-1,5-L-arabinan.

T Sakamoto1, J F Thibault.   

Abstract

An exo-arabinanase, designated Abnx, was purified from a culture filtrate of Penicillium chrysogenum 31B by ammonium sulfate precipitation, anion-exchange chromatography, and hydrophobic chromatography. Abnx had an apparent molecular mass of 47 kDa. The enzyme released only arabinobiose from the nonreducing terminus of alpha-1,5-L-arabinan and showed no activity towards p-nitrophenyl-alpha-L-arabinofuranoside and alpha-1,5-L-arabinofuranobiose. Abnx is the first enzyme with this mode of action.

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Year:  2001        PMID: 11425761      PMCID: PMC93020          DOI: 10.1128/AEM.67.7.3319-3321.2001

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


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