Literature DB >> 11425472

Molecular cloning and identification of UTR1 of a yeast Saccharomyces cerevisiae as a gene encoding an NAD kinase.

S Kawai1, S Suzuki, S Mori, K Murata.   

Abstract

UTR1 of the yeast Saccharomyces cerevisiae was cloned from the genomic DNA by polymerase chain reaction and expressed in Escherichia coli. Characterization of the purified UTR1p revealed that UTR1p is a NAD kinase consisting of six identical subunits with a molecular mass of 60 kDa. UTR1p specifically phosphorylated NAD in the presence of ATP, dATP, or CTP as phosphoryl donors, and was most active at pH 8.0, 30 degrees C. Km values of UTR1p for NAD and ATP were determined to be 0.50 mM and 0.60 mM, respectively.

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Year:  2001        PMID： 11425472     DOI： 10.1111/j.1574-6968.2001.tb10712.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  28 in total

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Authors:  Michael Y Galperin; Eugene V Koonin
Journal:  Nucleic Acids Res       Date:  2004-10-12       Impact factor: 16.971

2.  Evidence that feedback inhibition of NAD kinase controls responses to oxidative stress.

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3.  First archaeal inorganic polyphosphate/ATP-dependent NAD kinase, from hyperthermophilic archaeon Pyrococcus horikoshii: cloning, expression, and characterization.

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4.  Azo reductase activity of intact saccharomyces cerevisiae cells is dependent on the Fre1p component of plasma membrane ferric reductase.

Authors:  Patrícia A Ramalho; Sandra Paiva; A Cavaco-Paulo; Margarida Casal; M Helena Cardoso; M Teresa Ramalho
Journal:  Appl Environ Microbiol       Date:  2005-07       Impact factor: 4.792

Review 5.  Nuclear metabolism and the regulation of the epigenome.

Authors:  Ruben Boon; Giorgia G Silveira; Raul Mostoslavsky
Journal:  Nat Metab       Date:  2020-10-12

6.  Cloning and characterization of two NAD kinases from Arabidopsis. identification of a calmodulin binding isoform.

Authors:  William L Turner; Jeffrey C Waller; Barb Vanderbeld; Wayne A Snedden
Journal:  Plant Physiol       Date:  2004-07-09       Impact factor: 8.340

7.  POS5 gene of Saccharomyces cerevisiae encodes a mitochondrial NADH kinase required for stability of mitochondrial DNA.

Authors:  Micheline K Strand; Gregory R Stuart; Matthew J Longley; Maria A Graziewicz; Olivia C Dominick; William C Copeland
Journal:  Eukaryot Cell       Date:  2003-08

8.  A novel NADH kinase is the mitochondrial source of NADPH in Saccharomyces cerevisiae.

Authors:  Caryn E Outten; Valeria C Culotta
Journal:  EMBO J       Date:  2003-05-01       Impact factor: 11.598

9.  Identification of Ald6p as the target of a class of small-molecule suppressors of FK506 and their use in network dissection.

Authors:  Rebecca A Butcher; Stuart L Schreiber
Journal:  Proc Natl Acad Sci U S A       Date:  2004-05-14       Impact factor: 11.205

10.  Identification, molecular cloning and functional characterization of a novel NADH kinase from Arabidopsis thaliana (thale cress).

Authors:  William L Turner; Jeffrey C Waller; Wayne A Snedden
Journal:  Biochem J       Date:  2005-01-01       Impact factor: 3.857
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