Literature DB >> 11418581

New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants.

R Wagner1, M L Straub, J L Souciet, S Potier, J de Montigny.   

Abstract

The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine. A structure-function model for this PCP has been recently proposed. In this study, we developed a plasmid-based system that generated a number of affinity-mutated alleles, enabling us to define new amino acids critical for permease function.

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Year:  2001        PMID： 11418581      PMCID： PMC95330          DOI： 10.1128/JB.183.14.4386-4388.2001

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  13 in total

1.  Determination of a specific region of the purine-cytosine permease involved in the recognition of its substrates.

Authors:  J C Bloch; H Sychrova; J L Souciet; R Jund; M R Chevallier
Journal:  Mol Microbiol       Date:  1992-10       Impact factor: 3.501

2.  Amino acid residues N450 and Q449 are critical for the uptake capacity and specificity of UapA, a prototype of a nucleobase-ascorbate transporter family.

Authors:  C Meintanis; A D Karagouni; G Diallinas
Journal:  Mol Membr Biol       Date:  2000 Jan-Mar       Impact factor: 2.857

3.  Energy coupling in hypoxanthine transport of yeast. Potentiometric evidence for proton symport and potassium antiport.

Authors:  U Reichert; M Forêt
Journal:  FEBS Lett       Date:  1977-11-15       Impact factor: 4.124

4.  A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.

Authors:  A Baudin; O Ozier-Kalogeropoulos; A Denouel; F Lacroute; C Cullin
Journal:  Nucleic Acids Res       Date:  1993-07-11       Impact factor: 16.971

5.  Evidence for a dynamic role for proline376 in the purine-cytosine permease of Saccharomyces cerevisiae.

Authors:  T Ferreira; C Napias; J Chevallier; D Brèthes
Journal:  Eur J Biochem       Date:  1999-07

6.  Potassium transport by amino acid permeases in Saccharomyces cerevisiae.

Authors:  M B Wright; J Ramos; M J Gomez; K Moulder; M Scherrer; G Munson; R F Gaber
Journal:  J Biol Chem       Date:  1997-05-23       Impact factor: 5.157

7.  Trinucleotide insertions, deletions, and point mutations in glucose transporters confer K+ uptake in Saccharomyces cerevisiae.

Authors:  H Liang; C H Ko; T Herman; R F Gaber
Journal:  Mol Cell Biol       Date:  1998-02       Impact factor: 4.272

8.  Functional analysis of mutated purine-cytosine permease from Saccharomyces cerevisiae. A possible role of the hydrophilic segment 371-377 in the active carrier conformation.

Authors:  T Ferreira; D Brèthes; B Pinson; C Napias; J Chevallier
Journal:  J Biol Chem       Date:  1997-04-11       Impact factor: 5.157

9.  In vivo and in vitro studies of the purine-cytosine permease of Saccharomyces cerevisiae. Functional analysis of a mutant with an altered apparent transport constant of uptake.

Authors:  D Brèthes; M C Chirio; C Napias; M R Chevallier; J L Lavie; J Chevallier
Journal:  Eur J Biochem       Date:  1992-03-01

10.  Characterization of cytosine permeation in Saccharomyces cerevisiae.

Authors:  M R Chevallier; R Jund; F Lacroute
Journal:  J Bacteriol       Date:  1975-05       Impact factor: 3.490
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  5 in total

1.  Expression deconvolution: a reinterpretation of DNA microarray data reveals dynamic changes in cell populations.

Authors:  Peng Lu; Aleksey Nakorchevskiy; Edward M Marcotte
Journal:  Proc Natl Acad Sci U S A       Date:  2003-08-21       Impact factor: 11.205

2.  Inactivation of the FCY2 gene encoding purine-cytosine permease promotes cross-resistance to flucytosine and fluconazole in Candida lusitaniae.

Authors:  Florence Chapeland-Leclerc; Julien Bouchoux; Abdelhak Goumar; Christiane Chastin; Jean Villard; Thierry Noël
Journal:  Antimicrob Agents Chemother       Date:  2005-08       Impact factor: 5.191

3.  Molecular mechanisms of resistance to 5-fluorocytosine in laboratory mutants of Candida glabrata.

Authors:  Patrick Vandeputte; Laurent Pineau; Gérald Larcher; Thierry Noel; Daniel Brèthes; Dominique Chabasse; Jean-Philippe Bouchara
Journal:  Mycopathologia       Date:  2010-07-09       Impact factor: 2.574

4.  Identification of genes affecting hydrogen sulfide formation in Saccharomyces cerevisiae.

Authors:  Angela L Linderholm; Carrie L Findleton; Gagandeep Kumar; Yeun Hong; Linda F Bisson
Journal:  Appl Environ Microbiol       Date:  2008-01-11       Impact factor: 4.792

Review 5.  Saccharomyces cerevisiae and Caffeine Implications on the Eukaryotic Cell.

Authors:  Lavinia Liliana Ruta; Ileana Cornelia Farcasanu
Journal:  Nutrients       Date:  2020-08-13       Impact factor: 5.717
  5 in total

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