Literature DB >> 11418327

Two-dimensional electrophoresis and characterization of antigens from Paracoccidioides brasiliensis.

C A da Fonseca1, R S Jesuino, M S Felipe, D A Cunha, W A Brito, C M Soares.   

Abstract

Paracoccidioides brasiliensis is a fungal pathogen of humans. To identify antigens from P. brasiliensis we fractionated a crude preparation of proteins from the fungus and detected the IgG reactive proteins by immunoblot assays of yeast cellular extracts with sera of patients with paracoccidioidomycosis (PCM). We identified and characterized six new antigens by amino acid sequencing and homology search analyses with other proteins deposited in a database. The newly characterized antigens were highly homologous to catalase, fructose-1,6-biphosphate aldolase (aldolase), glyceraldehyde-3-phosphate dehydrogenase, malate dehydrogenase and triosephosphate isomerase from several sources. The characterized antigens presented preferential synthesis in yeast cells, the host fungus phase.

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Year:  2001        PMID： 11418327     DOI： 10.1016/s1286-4579(01)01409-5

Source DB:  PubMed          Journal:  Microbes Infect        ISSN： 1286-4579            Impact factor:   2.700


  27 in total

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2.  Glyceraldehyde-3-phosphate dehydrogenase of Paracoccidioides brasiliensis is a cell surface protein involved in fungal adhesion to extracellular matrix proteins and interaction with cells.

Authors:  Mônica Santiago Barbosa; Sônia Nair Báo; Patrícia Ferrari Andreotti; Fabrícia P de Faria; Maria Sueli S Felipe; Luciano dos Santos Feitosa; Maria José Soares Mendes-Giannini; Célia Maria de Almeida Soares
Journal:  Infect Immun       Date:  2006-01       Impact factor: 3.441

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Journal:  Eukaryot Cell       Date:  2007-11-26

7.  Comparison of virulence between Paracoccidioides brasiliensis and Paracoccidioides lutzii using Galleria mellonella as a host model.

Authors:  Liliana Scorzoni; Ana Carolina Alves de Paula e Silva; Junya de Lacorte Singulani; Fernanda Sangalli Leite; Haroldo Cesar de Oliveira; Rosangela Aparecida Moraes da Silva; Ana Marisa Fusco-Almeida; Maria José Soares Mendes-Giannini
Journal:  Virulence       Date:  2015-11-09       Impact factor: 5.882

8.  A secreted serine protease of Paracoccidioides brasiliensis and its interactions with fungal proteins.

Authors:  Juliana A Parente; Sílvia M Salem-Izacc; Jaime M Santana; Maristela Pereira; Clayton L Borges; Alexandre M Bailão; Célia M A Soares
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9.  Proteomic analysis reveals that iron availability alters the metabolic status of the pathogenic fungus Paracoccidioides brasiliensis.

Authors:  Ana F A Parente; Alexandre M Bailão; Clayton L Borges; Juliana A Parente; Adriana D Magalhães; Carlos A O Ricart; Célia M A Soares
Journal:  PLoS One       Date:  2011-07-28       Impact factor: 3.240

10.  Paracoccidoides brasiliensis 30 kDa adhesin: identification as a 14-3-3 protein, cloning and subcellular localization in infection models.

Authors:  Julhiany de Fatima da Silva; Haroldo César de Oliveira; Caroline Maria Marcos; Rosângela Aparecida Moraes da Silva; Tania Alves da Costa; Vera Lucia García Calich; Ana Marisa Fusco Almeida; Maria José Soares Mendes-Giannini
Journal:  PLoS One       Date:  2013-04-30       Impact factor: 3.240
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