Literature DB >> 11418108

In situ analysis of methylglyoxal metabolism in Saccharomyces cerevisiae.

A M Martins1, C A Cordeiro, A M Ponces Freire.   

Abstract

Methylglyoxal metabolism was studied during Saccharomyces cerevisiae grown with D-glucose as the sole carbon and energy source. Using for the first time a specific assay for methylglyoxal in yeast, metabolic fluxes of its formation and D-lactate production were determined. D-Glucose consumption and ethanol production were determined during growth. Metabolic fluxes were also determined in situ, at the glycolytic triose phosphate levels and glyoxalase pathway. Maximum fluxes of ethanol production and glucose consumption correspond to maxima of methylglyoxal and D-lactate formation fluxes during growth. Methylglyoxal formation is quantitatively related to glycolysis, representing 0.3% of the total glycolytic flux in S. cerevisiae.

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Year:  2001        PMID： 11418108     DOI： 10.1016/s0014-5793(01)02519-4

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  33 in total

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Journal:  Plant Signal Behav       Date:  2018-06-26

4.  Arginine 107 of yeast ATP synthase subunit g mediates sensitivity of the mitochondrial permeability transition to phenylglyoxal.

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Journal:  J Biol Chem       Date:  2018-08-09       Impact factor: 5.157

5.  Metabolic engineering of glycerol production in Saccharomyces cerevisiae.

Authors:  Karin M Overkamp; Barbara M Bakker; Peter Kötter; Marijke A H Luttik; Johannes P Van Dijken; Jack T Pronk
Journal:  Appl Environ Microbiol       Date:  2002-06       Impact factor: 4.792

6.  A H2O2-producing glyoxal oxidase is required for filamentous growth and pathogenicity in Ustilago maydis.

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8.  Sugar beet M14 glyoxalase I gene can enhance plant tolerance to abiotic stresses.

Authors:  Chuan Wu; Chunquan Ma; Yu Pan; Shilong Gong; Chenxi Zhao; Sixue Chen; Haiying Li
Journal:  J Plant Res       Date:  2012-12-01       Impact factor: 2.629

9.  Functional characterisation of glyoxalase I from the fungal wheat pathogen Stagonospora nodorum.

Authors:  Peter S Solomon; Richard P Oliver
Journal:  Curr Genet       Date:  2004-06-15       Impact factor: 3.886

10.  A comparative study of methylglyoxal metabolism in trypanosomatids.

Authors:  Neil Greig; Susan Wyllie; Stephen Patterson; Alan H Fairlamb
Journal:  FEBS J       Date:  2008-12-03       Impact factor: 5.542
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