Literature DB >> 11418029

Cloning and heterologous expression of a natural product biosynthetic gene cluster from eDNA.

S F Brady1, C J Chao, J Handelsman, J Clardy.   

Abstract

[reaction: see text] To study the natural products produced by uncultured microorganisms, an environmental DNA (eDNA) cosmid library was constructed and screened for the heterologous production of small molecules. A blue clone, CSL51, found in the eDNA library produces deoxyviolacein and the broad spectrum antibiotic violacein. The full sequence of the 6.7 kb eDNA violacein gene cluster and the characterization of violacein and deoxyviolacein from an eDNA clone are reported here.

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Year:  2001        PMID： 11418029     DOI： 10.1021/ol015949k

Source DB:  PubMed          Journal:  Org Lett        ISSN： 1523-7052            Impact factor:   6.005


  49 in total

1.  Novel antibacterial proteins from the microbial communities associated with the sponge Cymbastela concentrica and the green alga Ulva australis.

Authors:  Pui Yi Yung; Catherine Burke; Matt Lewis; Staffan Kjelleberg; Torsten Thomas
Journal:  Appl Environ Microbiol       Date:  2010-12-23       Impact factor: 4.792

Review 2.  Culture-independent discovery of natural products from soil metagenomes.

Authors:  Micah Katz; Bradley M Hover; Sean F Brady
Journal:  J Ind Microbiol Biotechnol       Date:  2015-11-19       Impact factor: 3.346

3.  Characterization of a novel amylolytic enzyme encoded by a gene from a soil-derived metagenomic library.

Authors:  Jiae Yun; Seowon Kang; Sulhee Park; Hyunjin Yoon; Myo-Jeong Kim; Sunggi Heu; Sangyeol Ryu
Journal:  Appl Environ Microbiol       Date:  2004-12       Impact factor: 4.792

4.  Phylogenetic screening of ribosomal RNA gene-containing clones in Bacterial Artificial Chromosome (BAC) libraries from different depths in Monterey Bay.

Authors:  M T Suzuki; C M Preston; O Béjà; J R de la Torre; G F Steward; E F DeLong
Journal:  Microb Ecol       Date:  2004-10-14       Impact factor: 4.552

Review 5.  Metagenomics: application of genomics to uncultured microorganisms.

Authors:  Jo Handelsman
Journal:  Microbiol Mol Biol Rev       Date:  2004-12       Impact factor: 11.056

Review 6.  Natural products from plant-associated microorganisms: distribution, structural diversity, bioactivity, and implications of their occurrence.

Authors:  A A Leslie Gunatilaka
Journal:  J Nat Prod       Date:  2006-03       Impact factor: 4.050

7.  Expression, crystallization and preliminary crystallographic data analysis of VioD, a hydroxylase in the violacein-biosynthesis pathway.

Authors:  Tingting Ran; Mengxiao Gao; Qiaoe Wei; Jianhua He; Lin Tang; Weiwu Wang; Dongqing Xu
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2015-01-28       Impact factor: 1.056

Review 8.  A functional approach to transcriptome profiling: linking gene expression patterns to metabolites that matter.

Authors:  Cindi A Hoover; Marc Slattery; Adam G Marsh
Journal:  Mar Biotechnol (NY)       Date:  2007-05-01       Impact factor: 3.619

9.  The violacein biosynthetic enzyme VioE shares a fold with lipoprotein transporter proteins.

Authors:  Katherine S Ryan; Carl J Balibar; Kaitlyn E Turo; Christopher T Walsh; Catherine L Drennan
Journal:  J Biol Chem       Date:  2008-01-02       Impact factor: 5.157

10.  Isolation of antibiotics turbomycin a and B from a metagenomic library of soil microbial DNA.

Authors:  Doreen E Gillespie; Sean F Brady; Alan D Bettermann; Nicholas P Cianciotto; Mark R Liles; Michelle R Rondon; Jon Clardy; Robert M Goodman; Jo Handelsman
Journal:  Appl Environ Microbiol       Date:  2002-09       Impact factor: 4.792
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