Affinity chromatography of DNA-binding proteins from human, murine and man-mouse hybrid cell lines.

About 60 proteins from human and murine cell lines were isolated by their ability to bind to different preparations of DNA. In the intact cell, the majority of these proteins are to be found in the cell nucleus. The electrophoretic mobilities of the DNA-binding proteins from human, murine and man-mouse hybrid cell lines were compared in two-dimensional acrylamide gels. Few, if any, species-specific differences were found. These observations suggest that the structures of the vast majority of the proteins that interact with DNA are conserved through evolution. A molecular basis is thus provided for the intracellular of hybrid cells derived from different animal species.