Literature DB >> 11413334

Viral replicase gene products suffice for coronavirus discontinuous transcription.

V Thiel1, J Herold, B Schelle, S G Siddell.   

Abstract

We have used vaccinia virus as a vector to clone a 22.5-kbp cDNA that represents the 5' and 3' ends of the human coronavirus 229E (HCoV 229E) genome, the HCoV 229E replicase gene, and a single reporter gene (coding for green fluorescent protein [GFP]) located downstream of a regulatory element for coronavirus mRNA transcription. When RNA transcribed from this cDNA was transfected into BHK-21 cells, a small percentage of cells displayed strong fluorescence. A region of the mRNA encoding GFP was amplified by PCR and shown to have the unique mRNA leader-body junction indicative of coronavirus-mediated transcription. These data show that the coronavirus replicase gene products suffice for discontinuous subgenomic mRNA transcription.

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Year:  2001        PMID： 11413334      PMCID： PMC114390          DOI： 10.1128/JVI.75.14.6676-6681.2001

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  28 in total

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Review 2.  Virus-encoded proteinases and proteolytic processing in the Nidovirales.

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3.  Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome.

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4.  The human coronavirus 229E superfamily 1 helicase has RNA and DNA duplex-unwinding activities with 5'-to-3' polarity.

Authors:  A Seybert; A Hegyi; S G Siddell; J Ziebuhr
Journal:  RNA       Date:  2000-07       Impact factor: 4.942

5.  High affinity interaction between nucleocapsid protein and leader/intergenic sequence of mouse hepatitis virus RNA.

Authors:  G W Nelson; S A Stohlman; S M Tahara
Journal:  J Gen Virol       Date:  2000-01       Impact factor: 3.891

6.  Retargeting of coronavirus by substitution of the spike glycoprotein ectodomain: crossing the host cell species barrier.

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9.  Subgenomic RNA synthesis directed by a synthetic defective interfering RNA of mouse hepatitis virus: a study of coronavirus transcription initiation.

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10.  Noncytopathic Sindbis virus RNA vectors for heterologous gene expression.

Authors:  E V Agapov; I Frolov; B D Lindenbach; B M Prágai; S Schlesinger; C M Rice
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Review 2.  The molecular biology of coronaviruses.

Authors:  Paul S Masters
Journal:  Adv Virus Res       Date:  2006       Impact factor: 9.937

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Authors:  Marcius S Almeida; Margaret A Johnson; Torsten Herrmann; Michael Geralt; Kurt Wüthrich
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5.  A complex zinc finger controls the enzymatic activities of nidovirus helicases.

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6.  Human coronavirus 229E papain-like proteases have overlapping specificities but distinct functions in viral replication.

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7.  Open reading frame 8a of the human severe acute respiratory syndrome coronavirus not only promotes viral replication but also induces apoptosis.

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Journal:  J Infect Dis       Date:  2007-06-19       Impact factor: 5.226

8.  Dodecamer structure of severe acute respiratory syndrome coronavirus nonstructural protein nsp10.

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9.  Coronavirus nucleocapsid protein facilitates template switching and is required for efficient transcription.

Authors:  Sonia Zúñiga; Jazmina L G Cruz; Isabel Sola; Pedro A Mateos-Gómez; Lorena Palacio; Luis Enjuanes
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10.  Nuclear magnetic resonance structure shows that the severe acute respiratory syndrome coronavirus-unique domain contains a macrodomain fold.

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Journal:  J Virol       Date:  2008-12-03       Impact factor: 5.103
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